废铁屑缓解硫化物抑制厌氧氨氧化脱氮性能的研究  

Performance of iron scrap to alleviate the inhibition of sulfides on denitrification by anaerobic ammoxidation

作　　者：陈洁[1] 王彩霞 王倩 沈耀良[1,2,3] 刘文如 CHEN Jie;WANG Cai-xia;WANG Qian;SHEN Yao-liang;LIU Wen-ru(School of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou 215000,China;National and Local Joint Engineering Laboratory for Municipal Sewage Resource Utilization Technology,Suzhou University of Science and Technology,Suzhou 215000,China;Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment,Suzhou 215000,China)

机构地区：[1]苏州科技大学环境工程学院,江苏苏州215000 [2]城市生活污水资源化利用技术国家地方联合工程实验室,江苏苏州215000 [3]江苏省水处理技术与材料协同创新中心,江苏苏州215000

出　　处：《中国环境科学》2023年第4期1636-1645,共10页China Environmental Science

基　　金：国家自然科学基金资助项目(51808367)。

摘　　要：在上流式厌氧污泥床反应器(UASB)中研究硫化物对厌氧氨氧化(Anammox)系统的长期抑制以及投加废铁屑后对系统性能的影响.结果表明:低浓度(<10mg/L)硫化物对系统无显著影响,当其浓度升至20,30,40mg/L时,使脱氮负荷(NRR)分别降低了13%,42%和51%;40mg/L硫化物使比厌氧氨氧化活性(SAA)降低了63%;Anammox细菌对硫化物抑制具有适应性;长期硫化物造成污泥颗粒结构恶化、上浮结块.投加废铁屑可以显著缓解硫化物抑制,使污泥沉降性能和系统脱氮效能快速恢复,19d后NRR比无硫阶段提高20%(达4.43kg/(m^(3)·d));SAA仅降低了16.8%.微生物群落表明,硫化物使Candidatus Kuenenia相对丰度从18.81%下降到7.31%,适应后丰度恢复,反应器底层丰度比顶层高出15倍;同时产生了反硝化菌(Arenimonas和Thiobacill).废铁屑投加后Candidatus Kuenenia丰度略有下降,顶层丰度高于底层;系统Thiobacillus丰度提高3.36倍,底层反硝化细菌的相对丰度上升.The long-term inhibition of sulfide on anaerobic ammonia oxidation(Anammox)system and the effect of adding scrap iron on the system performance were studied in an up-flow anaerobic sludge bed reactor(UASB).Results showed that low concentration(<10mg/L)of sulfide had no significant effect on the system performance.When its concentration increased to 20,30 and 40mg/L,the nitrogen removal rate(NRR)of the reactor decreased by 13%,42%and 51%,respectively.The high concentration(40mg/L)of sulfide reduced the specific anaerobic ammonia oxidation activity(SAA)by 63%.Anammox biomass had adaptability to the sulfide inhibition.Moreover,the long-term operation with sulfides caused the deterioration of particle structure,sludge floating,and caking.The addition of iron scrap significantly alleviated the inhibition of sulfide,and quickly recovered the sludge settleability and the reactor nitrogen removal efficiency.After 19 days of iron scrap addition,the NRR increased by 20%(up to 4.43kg/(m^(3)·d))compared with the sulfur-free stage,and the SAA reduced only by 16.8%.The microbial community analysis showed that the increased sulfide concentrations caused the decrease of the relative abundance of Candidatus Kuenenia from 18.81%to 7.31%.The relative abundance of Candidatus Kuenenia at the bottom layer of the reactor was 15 times higher than that at the top layer after adaptation.At the same time,the denitrifying bacteria(Arenimonas and Thiobacillus)were enriched.After adding scrap iron,the abundance of Ca.Kuenenia decreased slightly,but the relative abundance of Ca.Kuenenia at the top layer was higher than that at the bottom layer.The relative abundance of Thiobacillus in the system increased by 3.36 times,and the relative abundance of denitrifying bacteria in the bottom layer also increased.This study provides a new idea for promoting the application of Anammox process in sulfide containing wastewater.

关 键 词：ANAMMOX 硫化物 抑制 脱氮 废铁屑 

分 类 号：X703[环境科学与工程—环境工程]