寒旱地区填埋场调蓄池新鲜渗滤液稳定化影响因素  

The influencing factors of the stabilization of fresh leachate from landfill regulating ponds in cold and arid regions

作　　者：束寅慧 邵立明[1,2] 邓樱桃 吕凡 章骅[1,2] 何品晶 SHU Yin-hui;SHAO Li-ming;DENG Ying-tao;LÜFan;ZHANG Hua;HE Pin-jing(College of Environmental Science and Engineering,Tongji University,Shanghai 200092,China;Shanghai Institute of Pollution Control and Ecological Security,Shanghai 200092,China)

机构地区：[1]同济大学环境科学与工程学院,上海200092 [2]上海污染控制与生态安全研究院,上海200092

出　　处：《中国环境科学》2023年第4期1765-1772,共8页China Environmental Science

基　　金：国家重点研发计划项目(2018YFC1903700)。

摘　　要：为探索寒旱地区填埋场渗滤液实现生物稳定化的简便方法,本试验选择3种代表不同填埋龄期水质特征的渗滤液,在模拟调蓄池的厌氧环境中,按照4因素、3水平的正交规则,试验评估了渗滤液浓度(3.8,15.4,64.0g-COD/L)、污泥接种量(1,2,4g-VS/L)、搅拌频率(0,2,4次/h)、温度(10、20、30℃)4个因素对填埋场渗滤液生物稳定的影响.结果表明,渗滤液浓度和温度是影响稳定的主要因素.浓度最低(3.8g-COD/L)、温度最高(30℃)工况稳定时间最短(58d);浓度最高(64.0g-COD/L)工况仅在30℃下能在试验期(208d)内达到稳定.各试验组微生物群落结构分析表明,中低浓度(3.8和15.4g-COD/L)渗滤液各试验组古菌和细菌的群落结构基本相似,厌氧产甲烷代谢以Methanosaeta专营的乙酸营养型产甲烷途径为主;而高浓度(64.0g-COD/L)、30℃组达到稳定过程中,古菌和细菌群落发生明显演化,氢营养型产甲烷菌Methanofolli和Methanoculleus逐步富集且互营乙酸氧化菌属丰富的Firmicutes菌门的丰度增至80%以上,厌氧产甲烷以互营乙酸氧化产甲烷途径为主.依据试验结果分析,在调蓄池容量达到可容纳半年渗滤液产生量的条件下,在寒旱地区填埋场利用调蓄池稳定渗滤液具有应用可行性.To develop a viable and simple method to realize the bio-stabilization of landfill leachate in cold and arid regions,this experiment chose three kinds of leachate representing those with characteristics at different landfill ages.According to a 4-factor,and 3-level orthogonal rule,the study assessed the leachate concentration(3.8,15.4,64.0g-COD/L),sludge inoculation quantity(1,2,4g VS/L),agitation frequency(0,2,4 times/h),and temperature(10,20,30°C)on four aspects relating to landfill leachate bio-stabilization in the anaerobic environment of simulated regulating ponds.The findings demonstrated that the leachate concentration and temperature were the primary determinants of bio-stabilization.The lowest concentration(3.8g-COD/L)and highest temperature(30°C)condition had the shortest bio-stabilization time(58d).Analysis of the microbial community structure of each test group revealed that the bacterial and archaeal community structure were similar in each test group at low and medium concentrations(3.8 and 15.4g-COD/L),the acetoclastic methanogenesis pathway of Methanosaeta franchise dominated the methanogenesis route;while the archaeal and bacterial communities evolved significantly during the bio-stabilization process at high concentrations(64.0g-COD/L)and 30°C,and the hydrogenotrophic methanogenic bacteria Methanofolli and Methanoculleus were gradually enriched and the abundance of the phylum Firmicutes,which is rich in the genus relating to syntrophic acetate-oxidising Bacteria,increased to more than 80%,and the anaerobic methanogenesis was dominated by the tandem pathway of syntrophic acetate oxidation and hydrogenotrophic methanogenesis.Based on the analysis of the test results,it is feasible to stabilize leachate at landfills in cold and arid regions by using regulating ponds under the condition that the capacity of regulating ponds can accommodate a half-year leachate generation.

关 键 词：寒旱地区 调蓄池 渗滤液 厌氧生物降解 微生物群落结构 

分 类 号：X705[环境科学与工程—环境工程]