咪喹莫特对精子浓度、活动率及DNA碎片指数的影响  

Effect of imiquimod on sperm concentration,motility and DNA fragmentation index

作　　者：余宏亮 朱琛艳 刘艳丽 仵睛睛 YU Hongliang;ZHU Chenyan;LIU Yanli;WU Jingjing(Department of Andrology,Henan Institute of Reproductive Health Science and Technology,Henan Provincial Reproductive Obstetrics and Gynecology Hospital,National Health Council Key Laboratory of Birth Defect for Research and Prevention,Henan Key Laboratory of Population Defects Prevention,Zhengzhou,Henan 450002,China)

机构地区：[1]河南省生殖健康科学技术研究院、河南省生殖妇产医院、国家卫生健康委出生缺陷预防重点实验室、河南省人口缺陷干预技术研究重点实验室男科,河南郑州450002

出　　处：《安徽医药》2023年第5期1027-1030,共4页Anhui Medical and Pharmaceutical Journal

基　　金：河南省医学科技攻关计划(联合共建)项目(LHCJ20190826);河南省基本科研项目(JBKY2020013)。

摘　　要：目的 通过观察在培养液中加入咪喹莫特后精子浓度、活动率及DNA碎片指数(DFI)的变化,以明确免疫调节剂咪喹莫特是否会影响男性精子质量。方法 选取2021年9月至2022年1月河南省生殖妇产医院体检的健康男性志愿者新鲜液化的精液标本10份,每份分为不添加药物的空白对照组和咪喹莫特0.3、0.6、3.0μmol/L组。上游法培养60 min后分别检查四组上层精子浓度、活动率和精子DFI。结果 被测人员年龄(30.5±4.1)岁,精子浓度(48.82±24.43)×10^(9)/L,总活动率(50.83±23.33)%。培养后空白对照组精子浓度(23.53±15.45)×10^(9)/L,总活动率(16.54±16.04)%。培养后咪喹莫特组精子浓度(22.23±16.85)×10^(9)/L,精子总活动率(16.79±13.89)%。培养后各组精子浓度及活动率与培养前比较,差异有统计学意义(P<0.05);培养后各组精子浓度和活动率比较,差异无统计学意义(P>0.05)。培养后及各咪喹莫特剂量组均处于正常范围,与空白对照组比较,差异无统计学意义(P>0.05)。结论 当免疫调节剂咪喹莫特浓度在0.3~3.0μmol/L范围内时,对精子精子浓度、活动率及DFI等精子质量指标无明显影响。Objective To observe the changes of sperm concentration,motility and DNA fragmentation index(DFI)after adding a certain dose of imiquimod into the culture medium,in order to determine whether immunomodulator imiquimod has effect on male sperm quality.Methods A total of 10 freshly liquefied semen samples from healthy male volunteers who had physical examination in Henan Reproductive Maternity Hospital from September 2021 to January 2022 were selected.Fresh liquefied semen samples were assigned into imiquimod group and negative control group,imiquimod group includes 0.3,0.6,3.0μmol/L group.The upper sperm concentration,motility and sperm DFI of the four groups were examined after 60 min.Results The mean age was(30.5±4.1)years.Sperm concentration averaged was(48.82±24.43)×10^(9)/L;total motility was(50.8±23.33)%.After culture,the average concentration of sperm in the negative control group was(23.53±15.45)×10^(9)/L;total motility was(16.54±16.04)%.In imiquimod group,the sperm concentration was(22.23±16.85)×10^(9)/L,the mean total sperm motility was(16.79±13.89)%;the concentration and motility of each group were different before and after culture(P<0.05).There was no significant difference in sperm concentration and motility among the three groups of imiquimod(P>0.05).After culture and imiquimod dose groups were in the normal range,and the sperm DFI were no significant difference among the each dose of imiquimod group and the negative control group(P>0.05).Conclusion When the concentration of imiquimod was 0.3-3.0μmol/L,there was no significant effect on sperm concentration,motility and DFI.

关 键 词：咪喹莫特 精子 精液质量 DNA碎片指数 精液分析 

分 类 号：R698.2[医药卫生—泌尿科学]