“温脾涩肠方”隔药饼灸对克罗恩病的作用机制  

Mechanism of Herb-partitioned Moxibustion on Crohn′s Disease in Rats Following Spleen-warming and Intestine-astringing Formula

作　　者：冯骅[1] 丁敏[1] 蔡彬 张雪[1] 徐蕾[1] 戴文君[1] 易越 冯维琪 FENG Hua;DING Min;CAI Bin;ZHANG Xue;XU Lei;DAI Wenjun;YI Yue;FENG Weiqi(Wuxi Hospital Affiliated to Nanjing University of Chinese Medicine,Wuxi 214000,China)

机构地区：[1]南京中医药大学无锡附属医院,无锡214000

出　　处：《世界中医药》2023年第4期458-463,共6页World Chinese Medicine

基　　金：国家自然科学基金青年项目(82205110);江苏省研究生科研创新计划项目(KYCX20-15410);无锡市科技发展医疗卫生指导性计划项目(NZ2019024);无锡市“双百”中青年医疗卫生拔尖人才项目(HB2020060);南京中医药大学自然科学基金项目(XZR2021069)。

摘　　要：目的:探讨隔药饼灸对克罗恩病(CD)模型大鼠的作用机制,为隔药饼灸的临床应用提供科学依据。方法:将18只雄性SD大鼠随机分为正常对照组、模型对照组和隔药饼灸组,每组6只。模型采用2,4,6-三硝基苯磺酸(TNBS)灌肠法建立,隔药饼灸组用天枢(双)和气海穴隔药饼灸治疗14 d。采用结直肠扩张刺激(CRD)检测内脏敏感性。采用逆转录PCR和蛋白质印迹法分别检测磷脂酰肌醇3激酶(PI3K)、蛋白激酶B(AKT)和哺乳动物雷帕霉素靶蛋白(mTOR)以及下游自噬相关16样蛋白1(Atg16L1)、免疫相关鸟苷三磷酸酶M蛋白(IRGM)的mRNA和蛋白表达水平。结果:与正常对照组比较,模型对照组和隔药饼灸组的内脏疼痛阈值均显著下降(P<0.001)。干预后,与模型对照组比较,隔药饼灸组内脏疼痛阈值均显著上升(P<0.01),与正常对照组比较,隔药饼灸组内脏疼痛阈值差异无统计学意义(P>0.05)。与正常对照组比较,模型对照组的PI3K、AKT、mTOR、Atg16L1、IRGM蛋白表达量及mRNA表达量均明显升高,差异有统计学意义(均P<0.01);与模型对照组比较,隔药饼灸组的PI3K、AKT、mTOR、Atg16L1、IRGM蛋白表达及mRNA表达均明显降低,差异有统计学意义(均P<0.001)。结论:隔药饼灸可通过P13K/AKT/mTOR信号通路调节CD模型大鼠结肠组织自噬,提高内脏疼痛阈值,促进大鼠结肠溃疡组织恢复正常。Objective:To investigate the mechanism of herb-partitioned moxibustion(HPM) on Crohn′s disease(CD) in rats to provide a scientific basis for the clinical application of HPM.Methods:Eighteen male SD rats were randomly divided into a normal control group(NC),a model control group(MC),and an HPM group,with six rats in each group.The CD model was established by enema with 2,4,6-trinitrobenzenesulfonic acid(TNBS),and the rats in the HPM group were treated with HPM at Tianshu(ST 25,bilateral) and Qihai(CV 6) for 14 days.Colorectal distension(CRD) was used to detect visceral sensitivity.Reverse transcription(RT)-PCR and Western blot were used to detect the mRNA and protein expression of phosphoinositide 3-kinase(PI3K),protein kinase B(AKT),mammalian target of rapamycin(mTOR),autophagy-related 16-like 1(Atg16L1),and immunity-related GTPase family M protein(IRGM),respectively.Results:Compared with the NC group,the MC group and the HPM group showed decreased visceral pain thresholds(P0.001).After the intervention,compared with the MC group,the HPM group showed increased visceral pain threshold(P0.01).There was no difference in the visceral pain threshold between the HPM group and the NC group(P0.05).Compared with the NC group,the MC group showed increased protein and mRNA expression of PI3K,AKT,mTOR,Atg16L1,and IRGM(P0.01).Compared with the MC group,the HPM group showed decreased protein and mRNA expression of PI3K,AKT,mTOR,Atg16L1,and IRGM(P0.001).Conclusion:HPM can regulate the autophagy of colon tissues in CD rats and increase the visceral pain threshold by acting on the PI3K/AKT/mTOR signaling pathway.

关 键 词：隔药饼灸 克罗恩病 大鼠 自噬 PI3K/AKT/mTOR信号通路 内脏疼痛阈值 温脾涩肠 2 4 6-三硝基苯磺酸 

分 类 号：R256.3[医药卫生—中医内科学]