越冬期体色变异红罗非鱼差异表达miRNA的鉴定  

Identification of differentially expressed miRNA involved in skin color variation in red tilapia during overwintering

作　　者：王兰梅[1,2] 朱文彬 傅建军[1] 罗明坤[1] 董在杰 WANG Lanmei;ZHU Wenbin;FU Jianjun;LUO Mingkun;DONG Zaijie(Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture and Rural Affairs,Freshwater Fisheries Research Centre of Chinese Academy of Fishery Sciences,Wuxi 214081,China;Wuxi Fisheries College,Nanjing Agricultural University,Wuxi 214128,China)

机构地区：[1]中国水产科学研究院淡水渔业研究中心,农业农村部淡水渔业和种质资源利用重点实验室,江苏无锡214081 [2]南京农业大学无锡渔业学院,江苏无锡214128

出　　处：《水产学报》2023年第4期14-24,共11页Journal of Fisheries of China

基　　金：国家自然科学基金青年基金(31802290);江苏省自然科学基金面上项目(BK20221208);中国水产科学研究院基本科研业务费(2020TD37)。

摘　　要：为了探究红罗非鱼越冬期体色变异的分子机制,实验构建了其2种体色(正常粉红体色和变黑体色)皮肤组织的小RNA文库,每组4个重复,组装后平均每组分别获得12190544和11891890条过滤后的读长(clean reads),获得miRNA成熟序列669个,其中337个是已知的miRNAs。鉴定出越冬期变黑体色相对于正常粉红体色鱼的差异表达miRNAs 26个,其中上调11个,下调15个,且12个为已知的miRNAs,可能在红罗非鱼越冬期体色变异过程中发挥重要作用。富集分析发现了大量代谢和体色调控相关的通路。研究表明,红罗非鱼越冬期的体色变异可能是色素细胞的增殖和迁移形成的,与代谢相关的调控通路在其中发挥重要作用,为解析红罗非鱼越冬期体色变异的机制奠定了基础。Red tilapia(Oreochromis spp.)is a valuable fish due to its uniform red skin,the absence of black peritoneum,fast growth and adaptability to any culture system,and it has a huge market in many parts of the world,such as China,Malaysia and Thailand.However,the skin color differentiation in genetic breeding and skin color variation(whole pink to black)during the overwintering period are the main problems limiting the development of commercial red tilapia culture.MicroRNAs(miRNAs)play important roles in biological processes by regulating specific gene expression.Limited microRNA(miRNA)information is available on skin color variation in fish so far.In this study,a high-throughput Illumina sequencing of sRNAs was conducted on two color varieties of red tilapia during the overwintering.The two skin color varieties were normal whole pink(WP)and pink to black color(PB).A mean of 14748358 and 12981453 raw reads from WP and PB groups,respectively,corresponded to 12190544 and 11891890 clean reads.We summarized the length distribution of all samples and found that the peak length was 22 nt.A total of 669 mature miRNAs were identified,including 337 known miRNAs and 332 novel miRNAs.Moreover,we identified 26 significant differential expression miRNAs in PB compared with WP group,including 11 up-regulated miRNAs and 15 down-regulated miRNAs.And 12 known miRNAs,i.e.miR-203a,miR-200a,miR-200b,miR-725,miR-10544,miR-204a,miR-429b,miR-129,miR-10581c,miR-10581a,miR-217 and miR-499 may play an important role in the skin color variation of red tilapia during overwintering.Enrichment analysis found many pathways related to metabolic and skin color regulation including oocyte meiosis,Wnt,Notch,calcium,Apelin signaling pathway and so on.These results would provide a basis for exploring the mechanism of skin color variation of red tilapia during overwintering.

关 键 词：红罗非鱼 越冬 体色变黑 microRNA(miRNA) 

分 类 号：S965.12[农业科学—水产养殖]