Transfer of the zp3a gene results in changes in egg adhesiveness and buoyancy in transgenic zebrafish  

作　　者：Yu-Qing Cao Yong-Xin Wang Yan Zhao Jia Zhang Xian He Ping Xie Jun Chen Yu-Hua Sun 

机构地区：[1]Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,Hubei 430072,China [2]Innovation Academy for Seed Design,Chinese Academy of Sciences,Beijing 100049,China [3]University of Chinese Academy of Sciences,Beijing 100049,China [4]Donghu Experimental Station of Lake Ecosystems,State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,Hubei 430072,China

出　　处：《Zoological Research》2023年第2期259-268,共10页动物学研究（英文）

基　　金：supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB31000000)。

摘　　要：Reproductive strategies and spawning habits play key roles in the evolution of endemic East Asian cyprinids.However,the molecular mechanisms underlying the regulation of spawning habits are not well understood.We recently identified zona pellucida(Zp) as the top differentially expressed protein between East Asian cyprinids that produce adhesive and semi-buoyant eggs,suggesting that Zp protein may play important roles in the regulation of egg type.In this work,we generated transgenic zebrafish in which oocyte-specific expression of zp genes from rare minnow(Gobiocypris rarus),an East Asian cyprinid laying adhesive eggs,was driven by a zebrafish zp3.2 gene promoter.We found that the transgenic eggs obtained partial adhesiveness and exhibited alteration in hydration and buoyancy.Abnormal metabolism of vitellogenin(VTG) may contribute to enhanced hydration and/or buoyancy.Our work shows that expression of the exogenous zp3a gene from an adhesiveegg producing fish is sufficient to induce changes in both egg adhesiveness and buoyancy in zebrafish,emphasizing the important role of zp genes in the regulation of spawning habits.Our results thus provide new insights into how endemic East Asian cyprinids may have adapted to the Yangtze river-lake system via changes in spawning habits.

关 键 词：Zona pellucida protein ADHESIVE BUOYANCY HYDRATION Egg envelope 

分 类 号：Q953[生物学—动物学]