Intercross population study reveals that co-mutation of mitfa genes in two subgenomes induces red skin color in common carp(Cyprinus carpio wuyuanensis)  

作　　者：Bi-Jun Li Lin Chen Meng-Zhen Yan Xiao-Qing Zou Yu-Lin Bai Ya-Guo Xue Zhou Jiang Bao-Hua Chen Cheng-Yu Li Qian He Jian-Xin Feng Tao Zhou Peng Xu 

机构地区：[1]State Key Laboratory of Marine Environmental Science,College of Ocean and Earth Sciences,Xiamen University,Xiamen,Fujian 361102,China [2]Fujian Key Laboratory of Genetics and Breeding of Marine Organisms,College of Ocean and Earth Sciences,Xiamen University,Xiamen,Fujian 361102,China [3]College of Fisheries,Henan Normal University,Xinxiang,Henan 453007,China [4]Henan Academy of Fishery Science,Zhengzhou,Henan 450039,China

出　　处：《Zoological Research》2023年第2期276-286,共11页动物学研究（英文）

基　　金：supported by the National Key R&D Program of China (2019YFE0119000);National Natural Science Foundation of China (31872561);National Science Fund for Distinguished Young Scholars (32225049);Alliance of International Science Organizations (ANSO-CR-PP-2021-03)。

摘　　要：Common carp are among the oldest domesticated fish in the world.As such,there are many food and ornamental carp strains with abundant phenotypic variations due to natural and artificial selection.Hebao red carp(HB,Cyprinus carpio wuyuanensis),an indigenous strain in China,is renowned for its unique body morphology and reddish skin.To reveal the genetic basis underlying the distinct skin color of HB,we constructed an improved highfidelity(HiFi) HB genome with good contiguity,completeness,and correctness.Genome structure comparison was conducted between HB and a representative wild strain,Yellow River carp(YR,C.carpio haematopterus),to identify structural variants and genes under positive selection.Signatures of artificial selection during domestication were identified in HB and YR populations,while phenotype mapping was performed in a segregating population generated by HB×YR crosses.Body color in HB was associated with regions with fixed mutations.The simultaneous mutation and superposition of a pair of homologous genes(mitfa) in chromosomes A06 and B06 conferred the reddish color in domesticated HB.Transcriptome analysis of common carp with different alleles of the mitfa mutation confirmed that gene duplication can buffer the deleterious effects of mutation in allotetraploids.This study provides new insights into genotype-phenotype associations in allotetraploid species and lays a foundation for future breeding of common carp.

关 键 词：Cyprinus carpio Artificial selection COLORATION mitfa Structural variant 

分 类 号：Q953[生物学—动物学]