fruA基因对伤寒沙门菌侵袭力和生物膜形成的影响  

Effects of fruA gene on invasion and biofilm formation of Salmonella enterica serovar Typhi

作　　者：党娟娟 唐浩 靳梦彤 占子阳 汤新逸 张盈[1] 黄新祥[1] DANG Juanjuan;TANG Hao;JIN Mengtong;ZHAN Ziyang;TANG Xinyi;ZHANG Ying;HUANG Xinxiang(School of Medicine,Jiangsu University,Zhenjiang 212013,Jiangsu,China)

机构地区：[1]江苏大学医学院,江苏镇江212013

出　　处：《华中师范大学学报（自然科学版）》2023年第2期255-261,共7页Journal of Central China Normal University：Natural Sciences

基　　金：江苏省高等学校自然科学研究重大项目(20KJA310006);江苏省博士后科研资助计划项目(2021K209B)。

摘　　要：为探究fruA对伤寒沙门菌生长、毒力及生物膜形成的影响,利用pBAD33制备fruA的高表达菌株(WT-pfruA)及其空载体对照菌株(WT-pBAD33).通过细菌的生长曲线测定、泳动试验、T84细胞侵袭实验以及96孔微量板结晶紫染色法分别检测fruA对伤寒沙门菌生长、毒力及生物膜形成的影响;同时应用实时荧光定量PCR (qRT-PCR)分析fruA对伤寒沙门菌毒力及生物膜相关基因的调控作用.结果显示,成功制备了伤寒沙门菌的WT-pfruA及其WT-pBAD33,发现高表达fruA后,伤寒沙门菌的生长无明显变化、运动能力增强、侵袭力上升、生物膜形成能力增加;同样地,与WT-pBAD33相比,WT-pfruA中动力、侵袭及生物膜相关基因的转录水平明显上调.综上,fruA基因可增强伤寒沙门菌的毒力及生物膜形成,并对其相关表型基因起正调控作用.In order to investigate the effects of fruA on the growth,virulence and biofilm formation of Salmonella enterica serovar Typhi(S.Typhi),the overexpressing strain of fruA(WT-p fruA)and its empty vector strain(WT-pBAD33)were prepared by pBAD33.The effects of fruA on the growth,virulence and biofilm formation of S.Typhi were detected by bacterial growth curve assay,swimming assay,T84 cell invasion assay and 96-well microplate crystal violet staining.At the same time,qRT-PCR was used to analyze the regulation of fruA on the expression of virulence and biofilm related genes.The results showed that The WT-p fruA and WT-pBAD33 strains of S.Typhi were prepared successfully.After overexpression of fruA,the growth of S.Typhi did not change significantly,while the motility,invasion and biofilm formation ability increased.Similarly,compared with the control strain,the mRNA level of motility,invasion and biofilm related genes in WT-p fruA were significantly up-regulated.In conclusion,the fruA gene of S.Typhi was able to enhance the virulence and biofilm formation,and positively regulate its phenotype related genes.

关 键 词：伤寒沙门菌 FRUA 毒力 生物膜 

分 类 号：R378[医药卫生—病原生物学]