水飞蓟宾改善非酒精性脂肪性肝炎小鼠脂质代谢紊乱  

Silybin ameliorates lipid metabolism disorders in mice with non-alcoholic steatohepatitis

作　　者：蔡祖欢 邓桃妹 魏奶杰 朱丹[2] 钱斐 王广基[1] 张经纬[1] CAI Zuhuan;DENG Taomei;WEI Naijie;ZHU Dan;QIAN Fei;WANG Guangji;ZHANG Jingwei(Key Laboratory of Drug Metabolism&Pharmacokinetics,China Pharmaceutical University,Nanjing 210009,Jiangsu,China;Jiangsu Provincial Hospital of Chinese Medicine,Affiliated to Nanjing University of Chinese Medicine,Nanjing 210029,Jiangsu,China)

机构地区：[1]中国药科大学药物代谢动力学重点实验室,江苏南京210009 [2]南京中医药大学附属江苏省中医院,南京210029

出　　处：《中国临床药理学与治疗学》2023年第3期241-248,共8页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金面上项目(82173887);江苏省“六大人才高峰”项目(WSN-018)。

摘　　要：目的:研究水飞蓟宾对高脂高胆固醇(high-fat and high-cholesterol, HFHC)饮食诱导的非酒精性脂肪性肝炎(non-alcoholic steatohepatitis, NASH)小鼠肝脏脂质代谢的调控作用。方法:采用HFHC饮食喂养小鼠构建NASH模型,使用生化试剂盒检测血清中甘油三酯(triacylglycerol, TAG),总胆固醇(total cholesterol, T-CHO),低密度脂蛋白胆固醇(low-density lipoprotein cholesterol, LDL-C)和高密度脂蛋白胆固醇(high-density lipoprotein cholesterol, HDL-C)的水平。H&E染色和油红O染色用于检测肝组织病理学变化。运用脂质组学检测NASH小鼠肝脏各类脂质代谢的改变。结果:水飞蓟宾显著抑制NASH小鼠体重、肝重以及腹部脂肪的增加,降低血清T-CHO、TG和LDL-C的含量,改善肝脏脂滴蓄积和气球样变性,并回调NASH小鼠肝脏棕榈油酸(C16:1)和多不饱和长链脂肪酸(polyunsaturated long-chain fatty acids, PUFAs)的含量。结论:水飞蓟宾可能通过调节HFHC饮食诱导的小鼠肝脏脂质代谢异常从而减轻肝脏脂质蓄积和脂毒性。AIM:To investigate the regulatory effects of silybin on hepatic lipid metabolism in mice with non-alcoholic steatohepatitis(NASH)induced by high-fat and high-cholesterol(HFHD)diet.METHODS:Mice were fed a HFHD diet to construct a NASH model,and serum levels of triacylglycerol(TAG),total cholesterol(T-CHO),low-density lipoprotein cholesterol(LDL-C)and high-density lipoprotein cholesterol(HDL-C)were measured using biochemical kits.H&E staining and oil red O staining were used to detect histopathological changes in the liver.Lipidomics was used to detect the alterations of hepatic lipid metabolism in NASH mice.RESULTS:Silybin significantly inhibited the increase of body weight,liver weight and abdominal fat,decreased serum T-CHO,TAG and LDL-C levels,improved hepatic lipid droplet accumulation and ballooning degeneration,and back-regulated hepatic palmitoleic acid(C16:1)and polyunsaturated longchain fatty acids(PUFAs)in NASH mice.CONCLUSION:Silybin possibly reduced hepatic lipid accumulation and lipotoxicity by modulating abnormal hepatic lipid metabolism in mice induced by HFHC diet.

关 键 词：水飞蓟宾 非酒精性脂肪性肝炎 脂质代谢 

分 类 号：R285.5[医药卫生—中药学]