山楂黄酮抗动脉粥样硬化及降血脂作用机制研究  

Effects of hawthorn flavonoids on atherosclerotic and hyperlipidemia

作　　者：李军民[1] 牛恒立[1] 谢明全[1] 苏金龙[1] LI Junmin;NIU Hengli;XIE Mingquan;SU Jinlong(Pharmacy Department of the Second Hospital of Lanzhou University,Lanzhou 730000,Gansu,China)

机构地区：[1]兰州大学第二医院药学中心,甘肃兰州730000

出　　处：《中国临床药理学与治疗学》2023年第3期276-282,共7页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：甘肃省自然科学基金项目(22JR5RA982)。

摘　　要：目的:研究山楂黄酮对高血脂引起的动脉粥样硬化模型大鼠中的防治作用及作用机制。方法:采用高脂饲料+维生素D2的方式诱导主动脉粥样硬化大鼠模型,测定各组大鼠血脂水平,心脏指数、动脉粥样硬化指数(AI1,AI2)和冠心指数,通过油红O染色,HE染色,Masson染色观察大鼠主动脉组织病理学变化,利用ELISA方法,检测各组大鼠血清IL-6、细胞间黏附分子-1(ICAM-1)、单核细胞趋化蛋白-1(MCP-1)和血管细胞黏附分子-1(VCAM-1)的水平。结果:与正常组相较,动脉粥样硬化模型组大鼠总胆固醇(TC)、甘油三酯(TG)及低密度脂蛋白(LDL-C)、心脏指数、动脉粥样硬化指数(AI1,AI2)和冠心指数显著升高(P<0.01),高密度脂蛋白胆固醇(HDL-C)显著降低(P<0.01),主动脉病理评分明显增加,纤维化程度明显增加(P<0.01)。中、高剂量组山楂黄酮、阿托伐他汀组与模型组相较,TC、TG、LDL-C、心脏指数、动脉粥样硬化指数(AI1,AI2)和冠心指数显著降低(P<0.01),高密度脂蛋白胆固醇(HDL-C)显著升高(P<0.01),主动脉病理评分明显下降,纤维化程度明显改善(P<0.01)。在高血脂大鼠模型中观察到血脂水平变化趋势与此基本一致。同时与模型组相比,中、高剂量组山楂黄酮、阿托伐他汀组可显著抑制IL-6、MCP-1、ICAM-1和VCAM-1黏附分子的表达水平(P<0.01)。结论:山楂黄酮可抑制主动脉内皮动脉粥样硬化斑块的形成,降低小鼠动脉粥样硬化斑块的纤维化程度及炎症水平,达到抗动脉粥样硬化的目的,同时山楂黄酮具有调节血脂的作用。AIM:To investigate the preventive and therapeutic effects of Hawthorn flavone on hyperlipidemia and atherosclerosis rats.METHODS:The atherosclerosis model was established by high fat diet plus vitamin D2.The blood lipid levels,heart index,atherosclerosis index(AI1,AI2)and coronary heart index were measured in each group.The histopathological changes of aorta were observed by oil red O staining,HE staining and Masson staining.ELISA experiments were used to detect IL-6,ICAM-1,MCP-1 and VCAM-1 protein level.RESULTS:Compared with normal group,total cholesterol(TC),triglyceride(TG),low density lipoprotein(LDL-C),heart index,atherosclerosis index(AI1,AI2)and coronary index in atherosclerosis model group were significantly increased(P<0.01),while high density lipoprotein cholesterol(HDL-C)was significantly decreased(P<0.01).The pathological score of aorta and the degree of fibrosis were significantly increased(P<0.01).Compared with model group,TC,TG,LDL-C,heart index,atherosclerosis index(AI1,AI2)and coronary heart index were significantly decreased(P<0.01),and high-density lipoprotein cholesterol(HDL-C)was significantly increased(P<0.01)in medium,high dose hawthorn flavonoids and atorvastatin groups.The pathological score of aorta significantly decreased and the degree of fibrosis significantly improved(P<0.01).The variation trend of blood lipid levels in hyperlipidemia rats is basically consistent with atherosclerotic rats.Meanwhile,compared with model group,the medium,high dose hawthorn flavonoids and atorvastatin groups could significantly inhibit the expression levels of IL-6,MCP-1,ICAM-1 and VCAM-1 adhesion molecules(P<0.01).CONCLUSION:The hawthorn flavone can inhibit the formation of aortic endothelial atherosclerotic plaque,reduce the degree of fibrosis and inflammation of atherosclerotic plaque in rats,and achieve the purpose of anti-atherosclerosis.Meanwhile,the hawthorn flavone has the effect of regulating blood lipid.

关 键 词：山楂黄酮 动脉粥样硬化 血脂 炎症因子 

分 类 号：R541.4[医药卫生—心血管疾病] R965.2[医药卫生—内科学]