Introgression of Drought Tolerance into Elite Basmati Rice Variety through Marker-Assisted Backcrossing  

作　　者：Muhammad Sabar Shahzad Amir Naveed Shahid Masood Shah Abdul Rehman Khan Muhammad Musaddiq Shah Tahir Awan Muhammad Ramzan Khan Zaheer Abbas Muhammad Arif 

机构地区：[1]National Institute for Biotechnology and Genetic Engineering(NIBGE),Faisalabad,38000,Pakistan [2]Rice Research Institute,Kala Shah Kaku,39020,Pakistan [3]National Institute for Genomics and Advanced Biotechnology(NIGAB),Islamabad,44000,Pakistan [4]Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing,100081,China [5]Department of Biotechnology,COMSATS University Islamabad,Abbottabad,22020,Pakistan [6]Department of Biotechnology,University of Sialkot,Sialkot,51310,Pakistan [7]Pakistan Institute of Engineering and Applied Sciences,Islamabad,44000,Pakistan

出　　处：《Phyton-International Journal of Experimental Botany》2023年第5期1421-1438,共18页国际实验植物学杂志（英文）

基　　金：We thank Higher Education Commission of Pakistan and Green Super Rice Project a Component of Productivity Enhancement of Rice(PSDP No.0754),Pakistan for providing financial support.

摘　　要：Drought is one of the major abiotic threat to rice production in the context of climate change.Super Basmati is an elite,fine grain basmati rice variety grown in Punjab,Pakistan.Due to drought sensitive in nature,its yield has been facing an alarming situation in production because of gradual decrease in irrigated water for a couple of years.Three reported novel QTLs for drought tolerance were selected for incorporation into Super Basmati by employing marker assisted selection strategy.IR55419-04 with novel QTLs was used as a donor parent.Foreground selection was performed by applying PCR based QTL linked SSR markers followed by recombinant selection by using 2-4 flanking markers.Background selection was exercised by using polymorphic SSR markers for maximum genome recovery of the Super Basmati.The individuals homozygous at the target QTLs and with maximum background of Super Basmati at the rest of the non-target genome was selected for evaluation of drought tolerance.Under drought stress conditions,the yields of all introgressed lines(ILs)were 44.2%-125.7%higher than recurrent parent.Six superior ILs that are drought tolerant and very similar to Super Basmati in terms of agronomic and grain quality traits are marked for release as drought-tolerant varieties in arid regions or for use in breeding programs of high grain quality and drought-tolerant parents.

关 键 词：Drought QTL SSR markers basmati rice DROUGHT marker assisted breeding 

分 类 号：S511[农业科学—作物学]