十一味维命胶囊对抑郁症小鼠海马星形胶质细胞及谷氨酸代谢相关蛋白的影响  

Effects of Shi-Yi-Wei-Wei-Ming Capsule(十一味维命胶囊)on Hippocampal Astrocytes and Glutamate Metabolism Related Proteins in Depression Mice

作　　者：李鑫 王琼莹 孙洪昊 刘媛媛 约日古丽·麦海提 马召田 仁青东主[2] 任小巧 LI Xin;WANG Qiongying;SUN Honghao;LIU Yuanyuan;YUERI Guli·Maihaiti;MA Zhaotian;RENQING Dongzhu;REN Xiaoqiao(School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China;School of Tibetan Medicine,Qinghai University,Xining Qinghai 810016,China)

机构地区：[1]北京中医药大学中医学院,北京100029 [2]青海大学藏医药学院,青海西宁810016

出　　处：《中医药导报》2023年第3期30-35,共6页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：青海省重点研发与转化计划(2021-SF-C21):藏药十一味维命胶囊治疗“索隆病”的作用机制及临床疗效评价研究。

摘　　要：目的:探讨十一味维命胶囊对抑郁症小鼠海马星形胶质细胞及谷氨酸代谢相关蛋白的影响。方法:将36只小鼠随机分为正常组(n=9)、造模组(n=27)。造模组小鼠采用慢性不可预知温和应激(CUMS)结合慢性束缚应激(CRS)方法制备抑郁症小鼠模型。造模成功后,将小鼠随机分为模型组(n=9)、十一味维命胶囊组(n=9)、盐酸氟西汀组(n=9)。十一味维命胶囊组小鼠予十一味维命胶囊[234.0 mg/(kg·d)]灌胃,盐酸氟西汀组小鼠予盐酸氟西汀[5.2 mg/(kg·d)]灌胃,正常组、模型组小鼠予等体积生理盐水灌胃,干预28 d。采用糖水偏好实验、旷场实验及悬尾实验对小鼠进行行为学评估;免疫组化法检测小鼠海马CA1区GFAP的MOD值;Western Blotting法检测海马谷氨酸代谢相关蛋白GFAP、NMDAR2B、GS、EAAT1、EAAT2的表达。结果:造模21 d后,模型组、十一味维命胶囊组、盐酸氟西汀组小鼠体质量、糖水偏好率均低于正常组(P<0.01)。给药28 d后,模型组小鼠体质量、糖水偏好率显著低于正常组(P<0.01或P<0.05);十一味维命胶囊组、盐酸氟西汀组小鼠体质量、糖水偏好率均高于模型组(P<0.05)。造模21 d后,模型组、十一味维命胶囊组、盐酸氟西汀组小鼠悬尾不动时间均长于正常组(P<0.01)。给药28 d后,模型组小鼠悬尾不动时间显著长于正常组(P<0.01);十一味维命胶囊组、盐酸氟西汀组小鼠悬尾不动时间均短于模型组(P<0.05)。造模21 d后,模型组、十一味维命胶囊组、盐酸氟西汀组小鼠移动总距离、直立次数均低于正常组(P<0.01或P<0.05)。给药28 d后,模型组小鼠移动总距离、直立次数均低于正常组(P<0.01);十一味维命胶囊组、盐酸氟西汀组小鼠移动总距离、直立次数均高于模型组(P<0.01或P<0.05)。模型组小鼠海马CA1区GFAP的MOD值以及GFAP、NMDAR2B、GS、EAAT1、EAAT2蛋白相对表达量均显著低于正常组(P<0.01或P<0.05);十一味维命胶囊组、盐酸�Objective:To explore the effect of Shi-Yi-Wei-Wei-Ming capsule(SWMC)on hippocampal astrocytes and glutamate metabolism related proteins in depression mice.Methods:Totally 36 mice were randomly divided into normal group(n=9)and modeling group(n=27).The mouse model of depression was established by CUMS combined with CRS in the modeling group.After successful establishment of the model,the mice were randomly divided into model group(n=9),SWMC group(n=9)and fluoxetine hydrochloride group(n=9).The mice in the SWMC group were given SWMC[234.0 mg/(kg·d)]by intragastric administration.Fluoxetine hydrochloride group was given fluoxetine hydrochloride [5.2 mg/(kg·d)] by intragastric administration. Normal group and model group were given the same volume of normal saline by intragastric administration for 28 days. The behavior was evaluated by sucrose preference test, open field test and tail suspension test. The value of GFAP MOD in hippocampal CA1 region of mice was detected by immunohistochemical method. The expressions of glutamate metabolism related proteins, GFAP, NMDAR2B, GS, EAAT1 and EAAT2 in hippocampus were detected by Western blotting. Results: After 21 days of modeling, the model group, SWMC group and fluoxetine hydrochloride group showed lower body weight and sucrose preference rate than normal group (P<0.01). After 28 days of administration, the model group showed lower body weight and sucrose preference rate than normal group (P<0.01 or P<0.05). And the SWMC group and fluoxetine hydrochloride group showed higher body weight and sucrose preference rate than model group (P<0.05). After 21 days of modeling, the model group, SWMC group and fluoxetine hydrochloride group showed longer tail suspension immobility time than normal group (P<0.01). After 28 days of administration, the model group showed longer tail suspension immobility time than normal group (P<0.01). And the SWMC group and fluoxetine hydrochloride group showed shorter tail suspension immobility time than model group (P<0.01). After 21 days of mode

关 键 词：抑郁症 十一味维命胶囊 星形胶质细胞 谷氨酸-谷氨酰胺循环 盐酸氟西汀 小鼠 

分 类 号：R285.5[医药卫生—中药学]