癌胚抗原相关细胞黏附分子源性多肽KM17对血小板活化作用的研究  

Effect of CEACAM1-derived peptide KM17 on platelet activation

作　　者：万雯[1] 王华炜[1] 叶雨佳 李龙君[1] 杨理宏 杨晓娜 董玲 陈丽星[1] 孟照辉[1] Wan Wen;Wang Hua-wei;Ye Yu-jia;Li Long-jun;Yang Li-hong;Yang Xiao-na;Dong Ling;Chen Li-xing;Meng Zhao-hui(Department of Cardiology,The First Affiliated Hospital of Kunming Medical University,Kunming,Yunnan 650032,China)

机构地区：[1]昆明医科大学第一附属医院心内科,云南昆明650032

出　　处：《中国现代医学杂志》2023年第7期34-39,共6页China Journal of Modern Medicine

基　　金：国家自然科学基金(No:81860074);云南省教育厅科学研究基金项目(No:2022J0193)。

摘　　要：目的探讨癌胚抗原相关细胞黏附分子(CEACAM1)源性多肽KM17对血小板聚集、释放、黏附功能的影响。方法采用血小板聚集仪观察多肽KM17对凝血酶、胶原、花生四烯酸(AA)、二磷酸腺苷(ADP)等激动剂诱导的血小板聚集的影响;流式细胞术观察多肽KM17对ADP激活血小板后P-选择素释放的影响;显微镜下观察多肽KM17对血小板静态黏附于胶原基质的影响。结果多肽KM17能显著促进ADP诱导的血小板聚集且呈剂量依赖(P<0.05),而对AA、胶原及凝血酶诱导的血小板聚集差异均无统计学意义(P>0.05);此外,多肽KM17对ADP激活血小板后P-选择素释放及血小板静态黏附于胶原基质的差异也无统计学意义(P>0.05)。结论多肽KM17可明显促进ADP诱导的血小板聚集,但对ADP活化血小板后P-选择素释放及血小板静态黏附于胶原基质未见明显作用。Objective To explore the effect of KM17,a synthetic CEACAM1-derived peptide,on platelet aggregation,release,and adhesion.Methods We examined the effect of KM17 on platelet aggregation induced by different agonists(ADP,thrombin,collagen,and AA)with aggregometer.We observed the effect of KM17 on platelet P-selectin release after stimulated by ADP with FCM.The effect of KM17 on platelet adhesion to collagen was observed under a microscope.Results KM17 dose-dependently promoted ADP-induced platelet aggregation(P<0.05),but had no significant effect on platelet aggregation induced by AA,collagen,and thrombin(P>0.05).In addition,KM17 had no significant effect on platelet P-selectin release induced by ADP(P>0.05),and there was no significant difference in the numbers of platelet adhesion to collagen after incubated with different concentrations of KM17(P>0.05).Conclusions KM17 dose-dependently promoted platelet aggregation induced by ADP,but had no significant effect on platelet P-selectin release induced by ADP,and on platelet adhesion to collagen.

关 键 词：多肽KM17 血小板聚集 二磷酸腺苷 P-选择素 黏附 

分 类 号：R54[医药卫生—心血管疾病]