IL-10基因多态性与足月新生儿败血症易感性的相关性  

Association between IL-10 Gene Polymorphism and Susceptibility to Sepsis in Term Newborns

作　　者：赵晓芬[1] 杨米凤 赵朋娜 熊飞 朱双燕 张丽萍[1] ZHAO Xiao-fen;YANG Mi-feng;ZHAO Peng-na(Department of Neonatology,Kunming Children's Hospital,Yunnan Kunming 650228)

机构地区：[1]昆明市儿童医院新生儿科,云南昆明650228

出　　处：《医学临床研究》2023年第3期321-324,共4页Journal of Clinical Research

基　　金：国家自然科学基金地区科学基金项目(82060291);昆明市卫生健康委员会卫生科研课题(2020-06-03-001)。

摘　　要：【目的】探讨白细胞介素-10(IL-10)基因多态性与足月新生儿败血症易感性的相关性。【方法】前瞻性选取分析2020年6至2021年5月本院收治的107例汉族足月新生儿,根据是否确诊为败血症将其分为败血症组(败血症新生儿,56例)和对照组(咽下综合征、非感染性腹泻的新生儿,51例)。采用一代测序技术检测IL-10基因rs1800872、rs1800896多态性,比较两组基因型和等位基因频率的分布差异,采用Logistic回归分析IL-10基因rs1800872C/A、rs1800896G/A基因型与足月新生儿败血症易感性的相关性。【结果】败血症组血培养阳性15例,其中大肠埃希菌9例,葡萄球菌2例,溶血葡萄球菌、草绿色链球菌、无乳链球菌、屎肠球菌各1例。对照组血培养均为阴性。败血症组白细胞(WBC)计数及C反应蛋白(CRP)、降钙素原(PCT)水平均高于对照组,差异有统计学意义(P<0.05)。两组患儿IL-10基因rs1800872C/A、rs1800896G/A基因型及等位基因频率分布比较,差异均无统计学意义(P>0.05)。Logistic回归分析显示,IL-10基因rs1800872C/A、rs1800896G/A基因型与足月新生儿败血症的易感性无相关性(P>0.05)。【结论】IL-10基因rs1800872C/A、rs1800896G/A基因型可能不是足月新生儿败血症的易感基因。【Objective】To investigate the correlation between the polymorphisms of IL-10 the susceptibility to sepsis in term newborns.【Methods】A prospective analysis of the clinical data of 107 Han nationality term newborns admitted to our hospital from June 2020 to May 2021 was conducted.They were divided into sepsis group(56 newborns with sepsis)and control group(51 newborns with pharyngeal syndrome and non infectious diarrhea)based on whether they were diagnosed as sepsis.The polymorphism of the IL-10 rs1800872,rs1800896 gene was detected using first-generation sequencing technology,and the distribution differences of genotype and allele frequencies between the two groups were compared.Logistic regression was used to analyze the correlation between the IL-10 rs1800872C/A,1800896G/A genotypes and the susceptibility to sepsis in term infants.【Results】In the sepsis group,15 cases were positive for blood culture,including 9 cases of Escherichia coli,2 cases of Staphylococcus,1 case of Staphylococcus hemolyticus,1 case of Streptococcus viridis,1 case of Streptococcus lactis,and 1 case of Enterococcus faecium.The blood culture in the control group was negative.The WBC,serum CRP,and PCT levels in the sepsis group were significantly higher than those in the control group(P<0.05).There was no significant difference in the distribution of genotype and allele frequencies of IL-10 gene rs1800872C/A,rs1800896G/A between the two groups(P>0.05).Logistic regression analysis showed that there was no correlation between the genotype of IL-10 gene rs1800872C/A and rs1800896G/A and the susceptibility to sepsis in term newborns(P>0.05).【Conclusion】The IL-10 genes rs1800872C/A and rs1800896G/A may not be susceptible genes for sepsis in term newborns.

关 键 词：脓毒症 婴儿 新生 疾病 白细胞介素10/遗传 基因表达 多态现象 遗传 

分 类 号：R722.131[医药卫生—儿科]