X射线照射对小鼠小胶质细胞BV2炎症因子表达及细胞生物功能的影响  

Effect of X Ray Irradiation on Inflammatory Factors Expression and Biological Functions in BV2 Cells

作　　者：张海平 梁栋[3] 李娓 ZHANG Hai-ping;LIANG Dong;LI Wei(Department of Neurology and Neurosurgery,Xidian Group Hospital,Xi'an Shaanxi 710077)

机构地区：[1]陕西省西安市西电集团医院神经外科,陕西西安710077 [2]陕西省西安市西电集团医院神经内科,陕西西安710077 [3]中国科学院深圳先进技术研究院医药所,广东深圳518055

出　　处：《医学临床研究》2023年第3期332-336,共5页Journal of Clinical Research

基　　金：陕西省自然科学基金(编号:2019SF-179)。

摘　　要：【目的】探讨X射线照射对小鼠小胶质细胞BV2炎症因子表达及细胞生物功能的影响。【方法】8 Gy X射线照射小鼠小胶质细胞BV2后,采用反转录定量聚合酶链反应(RT-qPCR)和流式细胞仪检测小胶质细胞BV2炎症因子、M1型和M2型细胞表面受体表达情况;采用荧光标记颗粒和蛋白质印迹(Western blot)检测照射后小胶质细胞BV2吞噬能力、促分裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)信号通路蛋白水平;采用质谱分析照射后小胶质细胞BV2蛋白组学的改变情况。【结果】X射线照射后小胶质细胞BV2中白细胞介素1β(IL-1β)、肿瘤坏死因子-α(TNF-α)等炎症因子的mRNA水平显著增高,表面受体CD86表达增多,细胞吞噬能力增强且MAPK信号通路激活。【结论】X射线可引起小胶质细胞M1型极化,激活MAPK信号并促进炎症发生。【Objective】To explore the effects of X ray irradiation on inflammatory factors expression and biological function in mouse microglia cell line-BV2.【Methods】BV2 cells were irradiated by 8 Gy X ray.After irradiation,the expression of inflammatory factors,M1 and M2 cell surface markers were detected with RT-qPCR and flow cytometry.The phagocytic ability of irradiated BV2 cells were evaluated using fluorescein-labeled particles co-culture.The time course changes of MAPKs'(mitogen-activated protein kinase)phosphorylation in irradiated BV2 cells were investigated with Western blotting.Comparison of the proteomic difference between irradiated and unirradiated BV2 cells were performed by mass spectrum identification and bioinformatics analysis.【Results】The gene expression of inflammatory factors like IL-1β,TNF-αand the M1 cell surface marker CD86 were significantly upregulated in irradiated cells.Meanwhile,X ray irradiation enhanced BV2 cells'phagocytic ability and increased the phosphorylation level of MAPKs pathway.【Conclusion】X ray irradiation drives BV2 cells to M1-like activation and promotes inflammation through the phosphorylation of MAPK pathway.

关 键 词：X线/副作用 辐射损伤 实验性 小鼠 小神经胶质细胞/生理学 

分 类 号：R818[医药卫生—放射医学]