乙酰胆碱酯酶染色和钙视网膜蛋白免疫组化染色在先天性巨结肠患儿术前诊断中的应用价值  

Value of Acetylcholinesterase Staining and Calretinin Immunohistochemical Staining in the Preoperative Diagnosis of Hirschsprung's Disease in Children

作　　者：郑台青 姜楠[1] 文容 匡林芝 陈卫坚[1] ZHENG Tai-qing;JIANG Nan;WEN Rong(Hunan Children's Hospital,Changsha Hunan 410007)

机构地区：[1]湖南省儿童医院,湖南长沙410007

出　　处：《医学临床研究》2023年第3期378-380,384,共4页Journal of Clinical Research

摘　　要：【目的】探讨乙酰胆碱酯酶染色(AChE)和钙视网膜蛋白(Calretinin)免疫组化染色在先天性巨结肠(HD)患儿术前病理诊断中的价值。【方法】回顾性分析2021年4月至12月本院收治的89例慢性便秘、腹胀,或其他怀疑先天性巨结肠但症状不明显患儿的一般临床资料,其中HD患儿39例(HD组)和非HD患儿50例(非HD组)。分析Calretinin免疫组化染色、AChE染色在HD和非HD患儿中的特点,并分析两者诊断HD的准确性和敏感性。【结果】在HD组中AChE染色阳性为38例,阴性1例,而在非HD组中,AChE染色阳性为1例,阴性49例。HD组患儿Calretinin免疫组化染色均为阴性,而在非HD组患儿中,Calretinin免疫组化染色阳性为49例,阴性1例。AChE染色在HD的诊断中的特异度、灵敏度分别为97.4%、98.0%,Calretinin免疫组化染色在HD诊断中的灵敏度为100%,特异度为98%。【结论】结肠黏膜活检标本AChE染色联合Calretinin免疫组化染色是HD术前诊断的一种可靠的方法,值得临床推广应用。【Objective】To investigate the value of acetylcholinesterase(AChE)staining and Calretinin immunohistochemical staining in the preoperative pathological diagnosis of hirschsprung's disease(HD).【Methods】The general clinical data of 89 children with chronic constipation,abdominal distension,or other suspected congenital megacolon(but not obvious symptoms)admitted to our hospital from April to December 2021 were retrospectively analyzed,including 39 children with HD(HD group)and 50 children without HD(non HD group).The characteristics of Calretinin immunohistochemical staining and AChE staining in HD and non HD children were analyzed,and their accuracy and sensitivity in the diagnosis of HD were analyzed.【Results】In the HD group,AChE staining was positive in 38 cases and negative in 1 case,while in the non HD group,AChE staining was positive in 1 case and negative in 49 cases.In the HD group,the Caletinin immunohistochemical staining was negative,while in the non HD group,the Caletinin immunohistochemical staining was positive in 49 cases and negative in 1 case.The specificity and sensitivity of AChE staining in the diagnosis of HD were 97.4%and 98.0%,respectively.The sensitivity and specificity of Caletinin immunohistochemical staining in the diagnosis of HD were 100%and 98%,respectively.【Conclusion】The combination of AChE staining and Calretinin immunohistochemical staining in colonic mucosa biopsy specimens is a reliable method for preoperative diagnosis of HD,and is worthy of clinical application.

关 键 词：Hirschsprung病/诊断 乙酰胆碱酯酶 免疫组织化学 

分 类 号：R726.2[医药卫生—儿科]