青岛地区大白菜黑斑病病原菌分离鉴定与致病性研究  

Studies on Pathogenicity and Isolate Identification of Pathogen Causing Chinese Cabbage Black Spot in Qingdao Region

作　　者：宋雨萌 刘文杰 王敏 袁忠林 罗兰 SONG Yumeng;LIU Wenjie;WANG Min;YUAN Zhonglin;LUO Lan(Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management,College of Plant Health and Medicine,Qingdao Agricultural University,Qingdao 266109,Shandong,China)

机构地区：[1]青岛农业大学植物医学学院,山东省植物病虫害绿色防控工程研究中心,山东青岛266109

出　　处：《中国蔬菜》2023年第4期57-63,共7页China Vegetables

基　　金：“十三五”国家重点研发计划项目(2018YFD0201206-06)。

摘　　要：为明确山东省青岛地区大白菜黑斑病病原菌种类,2019—2020年从青岛市胶州市、莱西市、即墨区采集大白菜黑斑病病样18份,采用组织分离法分离病原菌,通过形态学观察结合分子生物学技术进行分类鉴定,并按照柯赫氏法则进行致病性测定。结果表明:从青岛地区采集的大白菜黑斑病病样中共分离到21株菌株,分别属于芸薹链格孢(Alternaria brassicae)、日本链格孢(A. japonica)、交链格孢(A. alternate),其中交链格孢为优势致病菌,检出率为76.2%;但致病性最强的为芸薹链格孢,病情指数为48.15。In order to clarify the kind of pathogen causing Chinese cabbage black spot in Qingdao region of Shandong Province,this experiment collected 18 accessions of sickened Chinese cabbage samples from Qingdao City Jiaozhou,Laixi and Jimo,in 2019—2020;isolated pathogenic fungi by tissue separation method;conducted classification and identification through morphological observation and molecular biological techniques;and carried out pathogenicity test according to Koch’s rule.The results showed that 21 strains were isolated from the sickened Chinese cabbage samples collected from Qingdao region,belonging to Alternaria brassicae,A.japonica and A.alternate,separately.Among them,A.alternate was the dominant pathogen with detection rate of 76.2%.But A.brassica was the most virulent with a disease index of 48.15.

关 键 词：大白菜黑斑病 链格孢菌 形态学观察 分子生物学鉴定 致病性 

分 类 号：S436.341.1[农业科学—农业昆虫与害虫防治]