喀什地区甜瓜田间杂草3种病毒的检测及分析  

Detection and Analysis of Three Viruses from Weeds in Melon Field of Kashi Area

作　　者：卡地尔阿依·买买提 李志卫 韩盛[2] 杨渡[2] 玉山江·麦麦提[2] 麦合木提江·米吉提 Kadierayi·Maimaiti;LI Zhiwei;HAN Sheng;YANG Du;Yushanjiang·Maimaiti;Maihemutijiang·Mijiti(College of Agriculture,Xinjiang Agricultural University,Key Laboratory of Pest Monitoring and Safety Control of Crops and Forests,Urumqi 830052,Xinjiang,China;Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Key Laboratory of Integrated Pest Management on Crops on Northwestern Oasis,Ministry of Agriculture and Rural Affairs,Urumqi 830091,Xinjiang,China;College of Life Science,Shanxi Normal University,Taiyuan 030002,Shanxi,China)

机构地区：[1]新疆农业大学农学院,农林有害生物监测与安全防控重点实验室,新疆乌鲁木齐830052 [2]新疆农业科学院植物保护研究所,农业农村部西北荒漠绿洲作物有害生物综合治理重点实验室,新疆乌鲁木齐830091 [3]山西师范大学生命科学学院,山西太原030002

出　　处：《中国蔬菜》2023年第4期64-69,共6页China Vegetables

基　　金：农业科技创新稳定支持项目(xjnkywdzc-2022004)。

摘　　要：2021年在新疆喀什地区伽师县和疏勒县厚皮甜瓜主产区随机采集了7科13种杂草的47份鲜样,采用RT-PCR法检测杂草带毒情况,分析杂草带毒率及病毒间的亲缘关系。结果表明:不同地点、不同种类杂草上的WMV、CMV、ZYMV检出率存在明显差异,伽师县WMV、CMV、ZYMV的检出率分别为5.77%、1.43%、32.19%,而疏勒县分别为17.14%、2.86%、7.14%;大部分杂草都携带病毒。杂草上的WMV、CMV和ZYMV存在较高的遗传变异。In 2021,total 47 fresh samples within 13 species from 7 families were randomly collected from the main producing areas of muskmelon in Jiashi and Shule counties of Xinjiang Kashi area.Virus carried of weeds were detected by RT-PCR method to analyze weed virus-carrying rate,and the relationship between virus.The results showed that there were significant differences among the detection rates of WMV,CMV and ZYMV in different locations and different kinds of weeds.The detection rates of WMV,CMV and ZYMV in Jiashi County were 5.77%,1.43%and 32.19%,respectively.While those in Shule County were 17.14%,2.86%and 7.14%,respectively.Most weeds carried viruses.There were higher genetic variation existed on weed WMV,CMV and ZYMV.

关 键 词：甜瓜 病毒病 田间杂草 RT-PCR检测 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]