Zr-30Ta和Zr-25Ta-5Ti烧结合金作为口腔科和骨科植入体的生物相容性和成骨活性  

Biocompatibility and osteogenic activity of Zr−30Ta and Zr−25Ta−5Ti sintered alloys for dental and orthopedic implants

作　　者：张毅能 杨海林[2] Akram Nasser JUAIM 陈晓娜 陆畅 邹玲 王寅舟 周雄文[3,4] Yi-neng ZHANG;Hai-lin YANG;Akram Nasser JUAIM;Xiao-na CHEN;Chang LU;Ling ZOU;Yin-zhou WANG;Xiong-wen ZHOU(The Fourth Hospital of Changsha City,Changsha 410006,China;State Key Laboratory of Powder Metallurgy,Central South University,Changsha 410083,China;National Clinical Research Center for Geriatric Disorders,Xiangya Hospital,Central South University,Changsha 410008,China;Department of Prosthodontics,Center of Stomatology,Xiangya Hospital,Central South University,Changsha 410008,China;Department of Prosthodontics,Faculty of Dentistry,Thamar University,Dhamar,Yemen;Department of Oral Implantology,Xiangya Stomatological Hospital&Xiangya School of Stomatology,Central South University,Changsha 410008,China)

机构地区：[1]长沙市第四医院,长沙410006 [2]中南大学粉末冶金国家重点实验室,长沙410083 [3]中南大学湘雅医院国家老年疾病临床研究中心,长沙410008 [4]中南大学湘雅医院口腔医学中心修复科,长沙410008 [5]Department of Prosthodontics,Faculty of Dentistry,Thamar University,Dhamar,Yemen [6]中南大学湘雅口腔医(学)院口腔种植科,长沙410008

出　　处：《Transactions of Nonferrous Metals Society of China》2023年第3期851-864,共14页中国有色金属学报（英文版）

基　　金：supported by the Natural Science Foundation of Hunan Province,China(No.2020JJ4732,2020JJ4933);the National Natural Science Foundation of China(No.52071343)。

摘　　要：采用细胞培养实验系统评价新型Zr-30Ta和Zr-25Ta-5Ti合金的生物相容性和成骨活性。采用轮廓仪和视频光学接触角测量仪测定合金的表面粗糙度和接触角。从蛋白质吸附和材料对MC3T3-E1成骨细胞、NIH3T3成纤维细胞和RAW264.7巨噬细胞的细胞毒性、黏附与增殖方面评价合金的生物学性能。通过碱性磷酸酶(ALP)活性、细胞外基质的矿化和胶原蛋白的分泌评价成骨活性。采用流式细胞术测定巨噬细胞极化以评估免疫反应。结果表明,Zr-30Ta和Zr-25Ta-5Ti合金的生物学性能优于CP-Ti合金,其成骨活性亦明显优于CP-Ti。在最初24 h内,Zr-30Ta和Zr-25Ta-5Ti合金能诱导巨噬细胞表型M1和M2的平衡表达。因Zr-30Ta和Zr-25Ta-5Ti合金良好的表面性能和化学成分,其具有更优的生物相容性和成骨活性。总之,Zr-30Ta和Zr-25Ta-5Ti合金是口腔科和骨科植入体的潜在候选材料。The biocompatibility and osteogenic activity of the newly developed Zr−30Ta and Zr−25Ta−5Ti alloys were systemically evaluated using cell culture experiments.The surface roughness and contact angle of the alloys were assessed using profilometer and drop-shape analyzer.The biological properties of the alloys were evaluated in terms of protein adsorption,cytotoxicity,adhesion,and proliferation of MC3T3-E1 osteoblasts,NIH3T3 fibroblasts,and RAW264.7 macrophages.The osteogenic activity was evaluated by means of the alkaline phosphatase(ALP)activity,extracellular matrix mineralization,and collagen secretion.The immune response was evaluated by determining the macrophage polarization on the samples using flow cytometry.The results indicated that Zr−30Ta and Zr−25Ta−5Ti alloys had better biocompatibility compared with commercially-pure titanium(CP-Ti).The osteogenic activity on the experimental alloys was significantly better than CP-Ti.Zr−30Ta and Zr−25Ta−5Ti alloys also induced a balanced expression of M1 and M2 macrophage phenotypes in the first 24 h.The favorable surface properties and chemical compositions of Zr−30Ta and Zr−25Ta−5Ti alloys were responsible for their improved biocompatibility and osteogenic activity.Overall,it is suggested that Zr−30Ta and Zr−25Ta−5Ti alloys are promising candidates for dental and orthopedic implant applications.

关 键 词：锆钽合金 生物相容性 成骨活性 种植体 巨噬细胞极化 

分 类 号：TG146.414[一般工业技术—材料科学与工程] R783.1[金属学及工艺—金属材料]