青光增视方对青光眼大鼠模型视网膜神经节细胞保护机制的研究  

The protective mechanism of Qingguang Zengshi on optic nerve injury in rats with glaucoma via the PI3K/AKT pathway

作　　者：姜艳华 赵磊[3] 陈琳琳[2] 左韬[1,3] JIANG Yanhua;ZHAO Lei;CHEN Linlin;ZUO Tao(Ophthalmology Department of Integrated Traditional Chinese and Western Medicine,The Second Clinical College of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China;Department of Ophthalmology,The Fourth People’s Hospital of Shenyang,China Medical University,Shenyang 110031,China;Department of Ophthalmology,The Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shen­yang 110034,China)

机构地区：[1]辽宁中医药大学第二临床学院中西医结合眼科,沈阳110032 [2]中国医科大学沈阳市第四人民医院眼科,沈阳110031 [3]辽宁中医药大学附属第二医院眼科,沈阳110034

出　　处：《中国医科大学学报》2023年第2期147-152,159,共7页Journal of China Medical University

基　　金：辽宁省自然科学基金(2018010548-301)。

摘　　要：目的探究青光增视方对青光眼大鼠模型视神经的保护作用及对PI3K/AKT通路的调节作用。方法通过烧灼大鼠右眼巩膜表面静脉制作青光眼模型,给予高、中、低剂量(3.40、1.70、0.85 g·kg^(-1)·d^(-1))青光增视方颗粒(青光增视方给药组)或甲钴胺(0.159 mg·kg^(-1)·d^(-1))(西药组)灌胃,末次给药后检测大鼠眼压,观察视网膜神经节细胞(RGCs)的形态及超微结构,采用定量PCR及Western blotting检测视网膜PI3K、AKT、PTEN、eNOS mRNA及蛋白的表达情况。结果与空白组比较,其他各组大鼠眼压均显著升高,但青光增视方高、中剂量组眼压显著低于模型组和西药组,差异均有统计学意义(均P<0.05)。与空白组比较,模型组PI3K、AKT、eNOS mRNA及PI3K、p-AKT/AKT、eNOS蛋白表达水平显著降低,PTEN mRNA及蛋白表达显著升高,差异均有统计学意义(均P<0.05)。与模型组比较,各给药组PI3K、AKT、e NOS mRNA及PI3K、p-AKT/AKT、eNOS蛋白表达水平显著升高,其中青光增视方给药组显著高于西药组;PTEN mRNA及蛋白表达水平显著降低,青光增视方给药组显著低于西药组,差异均有统计学意义(均P<0.05)。结论青光增视方对青光眼大鼠模型的RGCs具有保护作用,其机制可能与调控PI3K、AKT、PTEN、eNOS的表达相关。Objective To analyze the protective effect of Qingguang Zengshi on the optic nerve of rats with glaucoma and its effect on the phosphatidylinositol 3 kinase(PI3K)/protein kinase B(Akt)signaling pathway and its related factors.Methods Sprague Dawley rats were used to cauterize the scleral surface veins to create a right-eye glaucoma model.High,medium,and low doses(3.40,1.70,and 0.85 g·kg^(-1)·d^(-1))of Qingguang Zengshi(Qingguang Zengshi administration group)or methylcobalamin(0.159 mg·kg^(-1)·d^(-1),Western medicine group)were administered by gavage.Intraocular pressure was measured after the final administration.The morphology and ultrastructure of retinal ganglion cells were observed.Quantitative reverse transcription polymerase chain reaction and Western blotting were used to determine the mRNA and protein expression levels,respectively,of retinal PI3K,Akt,PTEN,and eNOS.Results Compared with the blank group,the intraocular pressure of rats in the other groups increased significantly,but was significantly lower in the high and middle dosage Qingguang Zengshi administration groups than that in the model group and the Western medicine group(P<0.05).Compared with the blank group,the expression levels of PI3K,AKT,and eNOS mRNA and PI3K,p-AKT/AKT,and eNOS proteins were significantly lower in the model group,and the expression levels of PTEN mRNA and protein were significantly higher in the model group(P<0.05).Compared with the model group,the expression levels of PI3K,AKT,and eNOS mRNA and PI3K,p-AKT/AKT,and eNOS proteins were significantly higher in each administration group,and were significantly higher in the Qingguang Zengshi administration group than the Western medicine group.The expression levels of PTEN mRNA and protein were significantly reduced,and were significantly lower in the Qingguang Zengshi administration group than the Western medicine group(P<0.05).Conclusion Qingguang Zengshi has protective effects on retinal ganglion cells in a rat model of glaucoma,and the mechanism may be related to the re

关 键 词：青光眼 视神经损伤 青光增视方 磷脂酰肌醇-3激酶 蛋白激酶B 

分 类 号：R289[医药卫生—方剂学]