Effect of radiation sterilisation on the structure and antibacterial properties of antimicrobial peptides  

作　　者：Xiaodan Wang Qinmei Li Huawei Yang 

机构地区：[1]Hygea Medical Technology Co.,Ltd.,Beijing,China [2]State Key Laboratory of Polymer Physics and Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun,Jilin Province,China [3]Department of Applied Chemistry and Engineering,University of Science and Technology of China,Hefei,Anhui Province,China

出　　处：《Biomaterials Translational》2023年第1期51-61,I0005,I0006,共13页生物材料转化电子杂志（英文）

基　　金：National Natural Science Foundation of China(No.51873213);High-Tech Research&Development Program of CAS-WEGO Group,and National Key Research and Development Program of China(No.2021YFC2101700).

摘　　要：Antimicrobial peptides(AMPs)have recently been exploited to fabricate anti-infective medical devices due to their biocompatibility and ability to combat multidrug-resistant bacteria.Modern medical devices should be thoroughly sterilised before use to avoid cross-infection and disease transmission,consequently it is essential to evaluate whether AMPs withstand the sterilisation process or not.In this study,the effect of radiation sterilisation on the structure and properties of AMPs was explored.Fourteen AMPs formed from different monomers with different topologies were synthesised by ring-opening polymerisation of N-carboxyanhydrides.The results of solubility testing showed that the star-shaped AMPs changed from water-soluble to water-insoluble after irradiation,while the solubility of linear AMPs remained unchanged.Matrix-assisted laser desorption/ionisation time of flight mass spectrometry showed that the molecular weight of the linear AMPs underwent minimal changes after irradiation.The results of minimum inhibitory concentration assay also illustrated that radiation sterilisation had little effect on the antibacterial properties of the linear AMPs.Therefore,radiation sterilisation may be a feasible method for the sterilisation of AMPs,which have promising commercial applications in medical devices.

关 键 词：antibacterial activity antimicrobial peptides radiation sterilisation 

分 类 号：TQ340[化学工程—化纤工业]