Effects of alendronate on bone mass and organ pathology in ovariectomized mice  

作　　者：WANG Long-fei GAO Yu-hai YANG Shi-chao HUANG Rui-xin LI Xing YANG Yutian XUAN Ying-ying TANG Han-qin CHEN Ke-ming 

机构地区：[1]College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China [2]Basic Medical Laboratory,No.940 Hospital of Joint Logistics Support Force of Chinese PLA,Lanzhou 730050,China [3]Gansu Key Laboratory of Stem Cells and Genetic Drug,Lanzhou 730050,China

出　　处：《Journal of Hainan Medical University》2023年第4期19-26,共8页海南医学院学报（英文版）

基　　金：General program of National Natural Science Foundation of China(81770879);Gansu Youth Science and Technology Fund Program(20JR5RA589);Joint Service Support Force 940 Hospital Laboratory Cultivation Project(2021yxky081)。

摘　　要：Objective:To investigate the effect of alendronate on bone mass and organ pathology of ovariectomized mice.Methods:Thirty SPF grade C57 female mice were randomly divided into three groups(n=10):Sham operation group(Sham),ovariectomized group(OVX)and ovariectomized+alendronate group(ALN).The sodium alendronate was injected subcutaneously at 400μg/kg twice a week in the ALN group.The equal volume of normal saline was injected subcutaneously twice a week in the SHAM group and OVX group.After 12 weeks of drug administration,the samples were taken.The organ coefficients,main organ pathological sections,and bone histopathological sections were observed,and the micro CT,L4 biomechanics and serum biochemical indicators were analyzed.Results:The uterine coefficient of Sham group was(0.0054±0.0007)significantly higher than that of OVX group(0.0026±0.0009)and ALN group(0.0025±0.0007),and the difference was statistically significant(P<0.05).No obvious lesions or toxic or side effects were observed in the main organs.Compared with the OVX group,the ALN group with decalcified sections of bone tissue had compact trabecular structure and fewer adipocytes.Micro-CT results showed that the Tb.BMD,Tb.N,Tb.Th and Tb.BV/TV values of the ALN group were significantly increased compared with those of the OVX group,but the Tb.Sp value was significantly decreased,and the difference was statistically significant(P<0.05).In L4 vertebral body biomechanics,the elastic modulus(50.29±13.43)and maximum load number(29.83±4.92)of ALN group were significantly higher than those of OVX group(14.77±3.12)and maximum load number(11.57±3.18),and the difference was statistically significant(P<0.05).Compared with the OVX group,the serum OCN and PINP indicators of bone formation in the ALN group were increased,while the bone resorption indicators TRACP-5b and CTX-I were decreased,with statistical significance(P<0.05).Conclusion:Alendronate sodium improves bone quality by increasing bone density,improving bone microstructure,increasing bone strength,p

关 键 词：Alendronate sodium Organ pathology Bone mineral density BIOMECHANICS Bone metabolism 

分 类 号：R580[医药卫生—内分泌]