Establishment and verification of theβ_(2)-AR desensitization asthma mice  

作　　者：ZHANG Yan SONG Gui-hua YU Su-ping LV Wei-gang GUO Yan-rong CHEN Xiao-song ZHANG Bing-xue ZHONG Hong-yun 

机构地区：[1]The First Affiliated Hospital of Henan University of TCM,Zhengzhou 450000,China

出　　处：《Journal of Hainan Medical University》2023年第4期33-37,共5页海南医学院学报（英文版）

基　　金：General Projects of National Natural Science Fund(81873338);Key Scientific and Technological Projects in Henan Province(202102310491);Training Subjects of Leading Talents in the Department of Traditional Chinese Medicine in Henan Province(Yuweizhongyihan[2021]No.8)。

摘　　要：Objective:To establish and verify aβ_(2)-AR desensitization asthma mice model.Methods:A total of 30 SPF male BALB/c mice were randomly divided into blank group,the common asthma group,andβ_(2)-AR desensitization asthma model group.Asthma model was established,and on this basis,the method of atom-izing inhalation and intraperitoneal injections of salbutamol was used to prepareβ_(2)-AR desensitization asthma model.After the last stimulation on the 21st day of modeling,the airway resistance of mice was measured.ELISA was used to detect the content of serum IgE;HE staining was used to observe the lung organization degree of infla-mmatory cell infiltration;Western blot method was used to detect theβ_(2)-AR content in lung tissue,RT-PCR was used to detect theβ_(2)-ARmRNA expressionin lung tissue.Results:Compared with the blank group,as acetyl choline(Mch)levels increased,groups of OVA induced airway resistance increases;but theβ_(2)-AR desensitization asthma model group increased airway resistance was more significant(P<0.05);compared with the blank group,IgE levels of common asthma group andβ_(2)-AR desensitization asthma model group elevated(P<0.01).The pathological histology observation found theβ_(2)-AR desensitization asthma airway inflammation infiltration in mice,the excessive mucus secretion and collagen deposition,and the pathological performance obviously increase compared with the common asthma group;β_(2)-AR content in the lung tissue ofβ_(2)-AR desensitization asthma model in mice,β_(2)-AR mRNA expression level in the blank group and common asthma model group were significantly decreased(P<0.05).Conclusion:Theβ_(2)-AR desensitization asthma mouse model was successfully established,and the buildingcycle was short.

关 键 词：Bronchial asthma Model β_(2)-AR desensitization 

分 类 号：R285.5[医药卫生—中药学]