不同泥水比ASBR颗粒污泥特性及微生物群落结构分析  

Analysis of sludge characteristics and microbial community structure of particles with different mud-water ratios as ASBR

作　　者：董建新 孙根行 王先宝 田利强 马琦 张子昂 DONG Jian-xin;SUN Gen-xing;WANG Xian-bao;TIAN Li-qiang;MA Qi;ZHANG Zi-ang(College of Environmental Science and Engineering,Shaanxi University of Science&Technology,Xi’an 710021,China)

机构地区：[1]陕西科技大学环境科学与工程学院,陕西西安710021

出　　处：《应用化工》2023年第3期701-707,共7页Applied Chemical Industry

基　　金：国家自然基金项目(52000126);陕西省重点研发计划项目(2022SF-234);温州市水污染控制与治理科技创新项目(S20160006)。

摘　　要：采用3套厌氧序批式反应器(ASBR),接种厌氧污泥,研究了不同泥水比(4∶6,5∶5,6∶4)R1、R2、R3反应器运行过程中有机物的去除效率及颗粒污泥性质的差异,同时利用高通量测序技术从分子生物学水平分析微生物群落结构。研究表明,3组反应器运行125 d,均培养出成熟的厌氧颗粒污泥,污泥粒径各范围(0.4~0.6,0.6~1.0,1.0~1.4和1.4~1.8 mm)的占比分别为12.5%,29.5%,49.8%,11.5%;10.4%,27.6%,46.4%,10.2%和9.8%,27.3%,40.1%,8.8%,R1培养出的颗粒污泥粒径较大,结构更为致密。扫描电镜发现,污泥菌群均由以丝状菌和杆状菌为主逐渐转变为以球状菌为主的集聚体。高通量测序结果表明,拟杆菌(Bacteroidetes)、绿弯菌门(Chloroflexi)是丰度较高的菌门;乙酸型产甲烷菌及氢营养型甲烷菌丰度大幅上升,分别由接种污泥时的29.7%和24.7%增长至52.9%,48.9%,52.2%和36.5%,33.8%和29.3%,泥水比较小的R1富集了更多的产甲烷菌,显著提升了甲烷产量。Three sets of anaerobic sequential batch reactors(ASBRs)were used to inoculate anaerobic sludge,and the removal efficiency of organic matter and the properties of granular sludge during the operation of R1,R2 and R3 reactors with different sludge-to-water ratios(4∶6,5∶5,6∶4)were studied,and the microbial community structure was analyzed from the molecular biology level by high-throughput sequencing technology.The results showed that the three groups of reactors ran for 125 d,and all of them cultivated mature anaerobic granular sludge,and the proportions of sludge particle size ranges(0.4~0.6,0.6~1.0,1.0~1.4 and 1.4~1.8 mm)were 12.5%,29.5%,49.8%and 11.5%,10.4%,27.6%,46.4%and 10.2%,9.8%,27.3%,40.1%and 8.8%,respectively.The granular sludge cultivated by R1 is larger in size and denser in structure.Scanning electron microscopy showed that the sludge flora gradually changed from filamentous bacteria and rod bacteria to aggregates dominated by globularia.The results of high-throughput sequencing showed that Bacteroidetes and Chloroflexi were highly abundant.The abundance of acetic acid methanogens and hydrotrophic methane bacteria increased significantly,from 29.7%and 24.7%when inoculating sludge to 52.9%,48.9%,52.2%,36.5%,33.8%and 29.3%,respectively.

关 键 词：厌氧序批式反应器 颗粒污泥性质 泥水比 微生物群落结构 

分 类 号：TQ033[化学工程] TQ110.5[环境科学与工程—环境工程] X703