Nuclear localization of human MEIOB requires its NLS in the OB domain and interaction with SPATA22  

作　　者：Yating Xu Zhen Chen Panfeng Wu Wei Qu Hanqing Shi Muyang Cheng Yumin Xu Tingyu Jin Cong Liu Cong Liu Yi Li Mengcheng Luo 

机构地区：[1]Hubei Provincial Key Laboratory of Developmentally Originated Disease,TaiKang Medical School(School of Basic Medical Sciences),Wuhan University,Wuhan 430071,China [2]Zhongnan Hospital of Wuhan University,Wuhan 430071,China [3]Center for Reproductive Medicine,Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou 510120,China

出　　处：《Acta Biochimica et Biophysica Sinica》2023年第1期154-161,共8页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China(No.31771588);the Strategic Collaborative Research Program of the Ferring Institute of Reproductive Medicine(No.FIRMC200509);the Key Research and Development Project of Hubei Province(No.2021BCA111);the Translational Medicine and Interdisciplinary Research(No.ZNJC201928)to M.L.

摘　　要：MEIOB is a vital protein in meiotic homologous recombination and plays an indispensable role in human gametogenesis.In mammals,MEIOB and its partner SPATA22 form a heterodimer,ensuring their effective localization on single-strand DNA(ssDNA)and proper synapsis processes.Mutations in human MEIOB(hMEIOB)cause human infertility attributed to the failure of its interaction with human SPATA22(hSPATA22)and ssDNA binding.However,the detailed mechanism is still unclear.In our study,truncated or full-length hMEIOB and hSPATA22 are traced by fused expression with fluorescent proteins(i.e.,copGFP or mCherry),and the live cell imaging system is used to observe the expression and localization of the proteins.When transfected alone,hMEIOB accumulates in the cytoplasm.Interestingly,a covered NLS in the OB domain of hMEIOB is identified,which can be exposed by hSPATA22 and is necessary for the nuclear localization of hMEIOB.When hSPATA22 loses its hMEIOB interacting domain or NLS,the nuclear localization of hMEIOB is aborted.Collectively,our results prove that the NLS in the OB domain of hMEIOB and interaction with hSPATA22 are required for hMEIOB nuclear localization.

关 键 词：MEIOB SPATA22 NLS OB domain protein interaction 

分 类 号：Q5[生物学—生物化学]