兔肝球虫与大肠埃希菌混合感染及耐药性分析  

Mixed Infection and Resistance Analysis of Rabbit Liver Cocci and E.Coli

作　　者：张昕 张自强[1] 段千禧 朱雪敏[1] 刘玉梅[1] ZHANG Xin;ZHANG Ziqiang;DUAN Qianxi;ZHU Xuemin;LIU Yumei(College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471000)

机构地区：[1]河南科技大学动物科技学院,洛阳471000

出　　处：《中国养兔》2023年第1期18-22,共5页Chinese Journal of Rabbit Farming

基　　金：河南省重点研发与推广专项(192102110077);河南省重点研发与推广专项(202102110093)。

摘　　要：兔肝球虫和大肠埃希菌是家兔养殖中常见且危害较大的疾病。为了确定河南省某兔场,家兔腹泻并大规模死亡的病原,在实验室对兔场发病兔进行病理剖检,寄生虫检测,采集组织样本进行病原菌的分离鉴定及药敏试验。结果显示:发病兔粪样检测到的球虫数量较少,未达到肠球虫感染标准;病理剖检发现个别兔肝脏有白色条形结节状结构;所有剖检兔胆汁镜检可见大量球虫卵囊。细菌分离培养得到一株革兰阴性杆状可疑致病菌;其eae基因序列扩增片段大小为833 bp,与大肠埃希菌KU058643.1相似性为99.7%;经药敏测定发现分离得到的大肠埃希菌同时对多黏菌素B、丁胺卡那、氨苄西林、头孢哌酮4种药物敏感,对其他药物有不同程度的耐药性。结论:导致此次兔场大规模发病死亡的主要原因是肝球虫和其继发的大肠埃希菌混合感染。试验结果为家兔养殖过程中肝球虫和大肠埃希菌混合感染的诊断以及临床用药提供了科学参考。Rabbit liver coccidia and Escherichia coli are common and harmful diseases in rabbit feeding.In order to determine the pathogen of diarrhea and mass death of rabbits in a rabbit farm in Henan Province,the laboratory carried out pathological autopsy,parasite detection,tissue collection for isolation and identification of pathogens and drug sensitivity test.The results showed that the number of coccidia detected in the feces of infected rabbits was less,which did not meet the standard of intestinal coccidia infection.Pathological examination showed that individual rabbit liver had white strip nodular structure.A large number of coccidia oocysts were found in bile microscopic examination of all dissected rabbits.A suspected gram-negative rod pathogen was isolated and cultured;The size of the amplified fragment of eae gene was 833 bp,and the similarity with E.coli KU 058643.1 was 99.7%.It was found that the isolated Escherichia coli was sensitive to polymyxin B,amikacin,ampicillin and cefoperazone,and had different degrees of resistance to other drugs.Conclusion:The main cause of large-scale morbidity and mortality in the rabbit farm was the mixed infection of hepatic coccidia and its secondary Escherichia coli.The results of this study provided a scientific reference for the diagnosis and clinical medication of mixed infection of hepatic coccidia and Escherichia coli in rabbits.

关 键 词：兔 球虫 大肠埃希菌 耐药性 

分 类 号：S829.1[农业科学—畜牧学]