2018-2020年甘肃省腹泻病毒流行特征及A组轮状病毒全基因组特征  

Epidemiological characteristics of viruses causing diarrhea and whole-genome features of rotavirus A in Gansu,2018-2020

作　　者：康倩[1] 吕彩霞 王雪莹 汪鹏[1] 张慧敏[1] 于德山[1] 李丹地[3] Kang Qian;Lyu Caixia;Wang Xueying;Wang Peng;Zhang Huimin;Yu Deshan;Li Dandi(Gansu Provincial Center for Disease Control and Prevention,Lanzhou 730030,Gansu,China;Yuzhong County Center for Disease Control and Prevention,Yuzhong 730100,Gansu,China;National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]甘肃省疾病预防控制中心,甘肃兰州730030 [2]榆中县疾病预防控制中心,甘肃榆中730100 [3]中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《疾病监测》2023年第2期170-176,共7页Disease Surveillance

基　　金：甘肃省青年科技基金计划(No.20JR5RA140);国家科技重大专项(No.2017ZX10103006)。

摘　　要：目的了解2018—2020年甘肃省A组轮状病毒(RVA)流行特征及全基因组特征。方法选取甘肃省腹泻症候群监测的6家哨点医院采集的腹泻患者粪便2553份,经实时聚合酶链式反应检测A组轮状病毒、诺如病毒、腺病毒、星状病毒、札如病毒核酸,选取部分轮状病毒阳性样本构建DNA测序文库,采用第二代测序,并进行分析。结果共检出病毒阳性标本853份,检出率33.41%,其中轮状病毒阳性率最高(17.43%),其次是诺如病毒(12.65%)、腺病毒(6.58%)、星状病毒(4.70%)、札如病毒(1.02%)。对50份轮状病毒阳性标本进行第二代测序,获得31株全序列,其中24株G9P[8]、2株G2P[8]、3株G2P[4]、1株G9P[6]和1株G9P[4]型别。全基因组核苷酸序列分析发现,除VP7片段外,序列差异比较大的有VP3和NSP4两个节段。本研究获得21株G9P[8]型Wa-like株(G9-P[8]-I1-R1-C1-M 1-A1-N1-T1-E1-H1),3株NSP4节段重配的G9P[8]-E2株(G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E2-H1),在测序的24株G9P[8]型RVA毒株中,G9P[8]-E2重配株占12.5%(3/24)。结论2018—2020年甘肃省轮状病毒是儿童腹泻的主要病原体,以G9P[8]基因型为绝对优势,首次发现G9P[8]-E2重配株,应持续进行病毒变异的监测。Objective To understand the circulation and whole-genome features of rotavirus A in Gansu province during2018-2020.Methods Stool samples were collected from children with diarrhea in 6 sentinel hospitals in Gansu for the extraction of viral nucleic acids of rotavirus A,adenovirus,norovirus,astrovirus and sapovirus with real-time PCR,and the DNA sequencing library of partial rotavirus positive samples were constructed following the instructions,the gene sequencing was conducted by the second generation sequencing method,and the sequencing data were analyzed by phylogenetic software.Results In the 2553 stool samples collected,853 were positive in nucleic acid test of adenovirus,norovirus,astrovirus,sapovirus and rotavirus(33.41%).The detection rate of rotavirus was highest(17.43%),followed by norovirus(12.65%),adenovirus(6.58%),astrovirus(4.70%)and sapovirus(1.02%).In the gene sequencing of 50 rotavirus positive samples,the full sequences of 31 strains were obtained,including 24 G9P[8]strains,2 G2P[8]strains,3 G2P[4]strains,1G9P[6]strain and 1 G9P[4]strain.Whole genome analysis suggested that except the VP7 segment,the VP3 and NSP4segments also had more obviously differences in all the 31 strains.This study obtained 21 G9P[8]Wa-like strains(G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1),3 G9P[8]-E2 strains(G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E2-H1),and G9P[8]-E2 strains accounted for12.5%(3/24)in 24 rotavirus G9P[8]strains sequenced.Conclusion Rotavirus was the major pathogen causing diarrhea in children in Gansu during 2018-2020,G9P[8]was the most predominant genotype,and G9P[8]-E2 reassortant strain was detected for the first time.It is necessary to continue the surveillance for virus variation.

关 键 词：A组轮状病毒 基因重配 第二代测序 G9P[8] 

分 类 号：R211[医药卫生—中医学] R117