应用ND-O-BSA-ELISA对贵州省贵阳市的麻风病患者家属及周围人群抗体水平的检测研究  

Application of ND-O-BSA-ELISA in detection of antibody level in family members and neighbors of leprosy patients in Guiyang,Guizhou

作　　者：杨琴[1] 佘敏[1] 张文月 周健[1] Yang Qin;She Min;Zhang Wenyue;Zhou Jian(Guiyang Municipal Center for Disease Control and Prevention,Guiyang 550051,Guizhou,China;Dermatology Hospital of Chinese Academy of Medical Sciences,Nanjing 210042,Jiangsu,China)

机构地区：[1]贵阳市疾病预防控制中心,贵州贵阳550051 [2]中国医学科学院皮肤病医院,江苏南京210042

出　　处：《疾病监测》2023年第2期207-210,共4页Disease Surveillance

基　　金：2019年度贵州省卫生健康委科学技术基金项目(No.gzwjkj2019-1-118)。

摘　　要：目的 了解贵州省贵阳市麻风患者家属及周围人群血清中麻风抗体水平情况,为麻风患者家属及周围人群的重点随访提供有力依据。方法 将检测对象的一般信息和应用ND-O-BSA-ELISA检测的IgM、MMP-IgG和LID-IgG抗体检测值导入Excel电子数据库,再用SPSS 18.0分析软件对抗体检测值进行χ^(2)检验和t检验。结果 麻风患者家属中3人的IgM为阳性,周围人群无阳性病例,但两组不同人群的IgM抗体变化差异无统计学意义(P>0.05);在麻风病患者家属组中,有50人MMPIgG为阳性,占23.47%,有44人LID-IgG为阳性,占20.66%;周围人群中有12人MMP-IgG阳性,占4.90%,11人LID-IgG阳性,占4.90%。两组不同人群的MMP-IgG和LID-IgG抗体变化差异具有统计学意义(P<0.001)。结论 应用ND-O-BSAELISA方法能有效检测麻风病患者家属及周围人群的麻风特异性抗体水平。在体检和随访时不仅要关心麻风病患者本人,也要关注其家属,同时兼顾周围人群。Objective To understand the serum antibody level of family members and neighbors of leprosy patients and provide evidence for the follow-up in family members and neighbor of leprosy patients. Methods The general information of the study subjects and the detection data of IgM, MMP-IgG and LID-IgG by ND-O-BSA-ELISA were entered into EXCEL electronic database, and then the detection data were analyzed by χ^(2) test and t test with software SPSS 18.0. Results Three family members of leprosy patients were IgM positive, no positive cases were detected in the neighbors, but there was no significant difference in the change of IgM between the two groups(P>0.05);In the family members of leprosy patients, 50were MMP-IgG positive, accounting for 23.47%, and 44 were LID-IgG positive, accounting for 20.66%. There were 12 MMPIgG positive cases, accounting for 4.90%, and 11 LID-IgG positive cases, accounting for 4.90%, in the neighbors of leprosy patients. The differences in changes of MMP-Ig G and LID-Ig G in the two groups were significant(P<0.001). Conclusion NDO-BSA-ELISA can effectively detect the level of leprosy specific antibody in the family members and neighbors of leprosy patients. In the physical examination and follow-up, attention should be paid not only to the patients, but also to their family members and neighbors.

关 键 词：ND-O-BSA-ELISA 麻风抗体 检测 

分 类 号：R211[医药卫生—中医学] R75R755