Evaluation of 1,4-naphthoquinone derivatives as antibacterial agents:activity and mechanistic studies  

作　　者：Zhizhuo Liu Zhemin Shen Shouyan Xiang Yang sun Jiahua Cui Jinping Jia 

机构地区：[1]School of Environmental Science&Engineering,Shanghai Jiao Tong University,Shanghai 200240,China [2]School of Chemistry and Chemical Engineering,Shanghai Jiao Tong University,Shanghai 200240,China

出　　处：《Frontiers of Environmental Science & Engineering》2023年第3期49-61,共13页环境科学与工程前沿（英文）

基　　金：the Startup Fund for Youngman Research at SJTU(No.19X100040082);the Medical and Engineering Interdisciplinary Research Fund of Shanghai Jiao Tong University(No.20X190020002).

摘　　要：The diverse and large-scale application of disinfectants posed potential health risks and caused ecological damage during the 2019-nCoV pandemic,thereby increasing the demands for the development of disinfectants based on natural products,with low health risks and low aquatic toxicity.In the present study,a few natural naphthoquinones and their derivatives bearing the 1,4-naphthoquinone skeleton were synthesized,and their antibacterial activity against selected bacterial strains was evaluated.In vitro antibacterial activities of the compounds were investigated against Escherichia coli and Staphylococcus aureus.Under the minimum inhibitory concentration(MIC)of≤0.125μmol/L for juglone(1a),5,8-dimethoxy-1,4-naphthoquinone(1f),and 7-methyl-5-acetoxy-1,4-naphthoquinone(3),a strong antibacterial activity against S.aureus was observed.All 1,4-naphthoquinone derivatives exhibited a strong antibacterial activity,with MIC values ranging between 15.625 and 500μmol/Land ECso values rangingbetween10.56 and248.42μmol/L.Most of the synthesized compounds exhibited strong antibacterial activities against S.aureus.Among these compounds,juglone(la)showed the strongest antibacterial activity.The results from mechanistic investigations indicated that juglone,a natural naphthoquinone,caused cell death by inducing reactive oxygen species production in bacterial cells,leading to DNA damage.In addition,juglone could reduce the self-repair ability of bacterial DNA by inhibiting RecA expression.In addition to having a potent antibacterial activity,juglone exhibited low cytotoxicity in cell-based investigations.In conclusion,juglone is a strong antibacterial agent with low toxicity,indicating that its application as a bactericidal agent may be associated with low health risks and aquatic toxicity.

关 键 词：1 4-naphthoquinone derivatives ANTIBACTERIAL Actionmechanism RECA 

分 类 号：TQ465[化学工程—制药化工]