Tank-dependence of the functionality and network differentiation of activated sludge community in a full-scale anaerobic/anoxic/aerobic municipal sewage treatment plant  

作　　者：Hongcan Cui Ronghua Xu Zhong Yu Yuanyuan Yao Shaoqing Zhang Fangang Meng 

机构地区：[1]School of Environmental Science and Engineering,Sun Yat-sen University,Guangzhou 510275,China [2]Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology,Sun Yat-sen University,Guangzhou 510275,China

出　　处：《Frontiers of Environmental Science & Engineering》2023年第3期107-120,共14页环境科学与工程前沿（英文）

基　　金：the National Key R&D Program of China(No.2017YFE0114300);National Natural Science Foundation of China(Nos.32161143031,51622813 and 51878675).

摘　　要：Understanding the structures and dynamics of bacterial communities in activated sludge(AS)in full-scale wastewater treatment plants(WWTPs)is of both engineering and ecological significance.Previous investigations have mainly focused on the AS communities of WWTP aeration tanks,and the differences and interactions between the communities in anaerobic and anoxic tanks of the AS system remain poorly understood.Here,we investigated the structures of bacterial communities and their inter-connections in three tanks(anaerobic,anoxic,and aerobic)and influent from a full-scale WWTP with conventional anaerobic/anoxic/aerobic(A/A/O)process over a year to explore their functionality and network differentiation.High-throughput sequencing showed that community compositions did not differ appreciably between the different tanks,likely due to the continuous sludge community interchange between tanks.However,network analysis showed significant differences in inter-species relationships,OTU topological roles,and keystone populations in the different AS communities.Moreover,the anoxic network is expected to be more unstable and easily affected by environmental disturbance.Tank-associated environmental factors,including dissolved oxygen,pH,and nutrients,were found to affect the relative abundance of functional genera(i.e.,AOB,NOB,PAOs,and denitrifiers),suggesting that these groups were more susceptible to environmental variables than other bacteria.Therefore,this work could assist in improving our understanding of tank-associated microbial ecology,particularly the response of functional bacteria to seasonal variations in WWTPs employing A/A/O process.

关 键 词：Activated sludge Bacterial community Tank-dependence Network association Functional bacteria 

分 类 号：X703[环境科学与工程—环境工程]