银杏叶提取物预处理联合肢体远隔缺血后处理对缺血再灌注损伤大鼠氧化应激反应及心肌组织中热休克蛋白70表达的影响  

Effect of extract of ginkgo biloba leaf pretreatment combined with limb remote ischemic postconditioning on oxidative stress response and the expression of heat shock protein 70 in myocardial tissue of rats with ischemia-reperfusion injury

作　　者：吴蕊 闫菲 韩雅琪 王文涛 王吉静 WU Rui;YAN Fei;HAN Yaqi;WANG Wentao;WANG Jijing(Department of Heart Center,Fuwai Central China Cardiovascular Hospital/Henan Provincial People′s Hospital,Zhengzhou 450000,Henan Province,China;Department of Cardio-Pulmonary Function,Henan Provincial People′s Hospital,Zhengzhou 450000,Henan Province,China)

机构地区：[1]阜外华中心血管病医院/河南省人民医院心脏中心,河南郑州450000 [2]河南省人民医院心肺功能科,河南郑州450000

出　　处：《新乡医学院学报》2023年第4期301-307,共7页Journal of Xinxiang Medical University

基　　金：河南省医学科技攻关(联合共建)项目(编号:LHGJ20190782,LHGJ20200089)。

摘　　要：目的探讨银杏叶提取物(EGB)预处理和肢体远隔缺血后处理(RIP)对缺血再灌注(IR)大鼠氧化应激反应及心肌组织中热休克蛋白70(HSP70)表达的影响。方法将60只雄性大鼠随机分为假手术组、IR组、EGB预处理组、RIP组和联合处理组,每组12只。假手术组大鼠用丝线穿过冠状动脉左前降支但不结扎;其余各组大鼠结扎冠状动脉左前降支制备IR损伤模型。EGB预处理组大鼠造模前30 min腹腔注射EGB 50 mg·kg^(-1);RIP组大鼠在左前降支结扎15 min时实施RIP,不给予药物治疗;联合处理组大鼠造模前30 min腹腔注射EGB 50 mg·kg^(-1),造模前15 min实施RIP;IR组大鼠造模前30 min腹腔注射与EGB等体积的生理盐水。假手术组大鼠关闭胸腔3 h后经右颈内静脉采血4 mL,其余各组大鼠在恢复血流再灌注后3、12、24、48 h经右颈内静脉采血4 mL,采用比色法检测各组大鼠血清中丙二醛(MDA)和超氧化物歧化酶(SOD)水平。采血后处死大鼠,采用免疫组织化学法检测各组大鼠心肌组织中HSP70的相对表达量。结果再灌注3 h时,IR组、EGB预处理组、联合处理组与假手术组大鼠血清中MDA水平比较差异无统计学意义(P>0.05),RIP组大鼠血清中MDA水平显著高于假手术组(P<0.05);其余各时间点,IR组、EGB预处理组、RIP组和联合处理组大鼠血清中MDA水平显著高于假手术组(P<0.05)。IR组、EGB预处理组、RIP组、联合处理组大鼠各时间点血清中SOD水平均显著高于假手术组(P<0.05)。再灌注3 h时,EGB预处理组、RIP组大鼠血清中MDA、SOD水平与IR组比较差异无统计学意义(P>0.05);联合处理组大鼠血清中MDA水平显著低于IR组,SOD水平显著高于IR组(P<0.05)。再灌注24 h时,联合处理组大鼠血清中MDA水平显著低于EGB预处理组(P<0.05);其余各时间点,联合处理组与EGB预处理组大鼠血清中MDA水平比较差异均无统计学意义(P>0.05)。再灌注48 h时,联合处理组大鼠血清中SOD水平显著Objective To inrestigate the effect of extract of ginkgo biloba leaf(EGB)pretreatment combined with limb remote ischemic postconditioning(RIP)on oxidative stress response and the expression of heat shock protein 70(HSP70)in myocardial tissue of rats with ischemia reperfusion(IR)injury.Methods Sixty male rats were randomly divided into sham operation group,IR group,EGB pretreatment group,RIP group and combined treatment group,with 12 rats in each group.The rats in the sham operation group were threaded through the left anterior descending branch of the coronary artery without ligation;the rats in another groups were ligated the left anterior descending branch of the coronary artery to prepare the IR injury model.The rats in the EGB pretreatment group were intraperitoneally injected with EGB 50 mg·kg^(-1) at 30 min before modeling;the rats in the RIP group were given RIP at 15 min after ligating the left anterior descending,and were not given drug treatment;the rats in the combined treatment group were intraperitoneally injected with EGB 50 mg·kg^(-1) at 30 min before modeling,and were given RIP at 15 min before modeling;the rats in the IR group were intraperitoneally injected with the same volume of normal saline as EGB at 30 min before modeling.In the sham operation group,4 mL of blood was collected from the right internal jugular vein of rats at 3 hours after the thoracic cavity was closed;in the another groups,the 4 mL of blood was collected from the right internal jugular vein of rats at 3,12,24 and 48 hours after the blood flow was restored;and the serum malondialdehyde(MDA),superoxide dismutase(SOD)levels of rats in each group were measured by colorimetry.The rats were killed after blood collection to take myocardial tissue,and the relative expression of HSP70 in myocardial tissue of rats in each group was detected by immunohistochemistry.Results At 3 h after reperfusion,there was no significant difference in serum MDA level of rats between the IR group,EGB pretreatment group,combined treatment group and t

关 键 词：缺血再灌注损伤 肢体远隔缺血后处理 银杏叶提取物 热休克蛋白70 

分 类 号：R541[医药卫生—心血管疾病]