培养条件对雪茄烟优势菌株ZLX10生长的影响  

Effects of Culture Conditions on the Growth of Dominant Strain ZLX10 from Cigar

作　　者：潘勇[1] 张贝贝 周亚彬 胡捷[1] 景玉辉 李林林 黄友谊[3] 王剑[1] PAN Yong;ZHANG Beibei;ZHOU Yabin;HU Jie;JING Yuhui;LI Linlin;HUANG Youyi;WANG Jian(Hubei Zhongyan Industry Co.,Ltd.,Wuhan 430040,China;College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430077,China;College of Horticulture and Forestry,Huazhong Agricultural University,Wuhan 430077,China)

机构地区：[1]湖北中烟工业有限责任公司,湖北武汉430040 [2]华中农业大学生命科学技术学院,湖北武汉430077 [3]华中农业大学园艺林学学院,湖北武汉430077

出　　处：《广东农业科学》2023年第2期11-19,共9页Guangdong Agricultural Sciences

基　　金：湖北中烟工业有限责任公司科技项目(2021JCYL3SX2B011)。

摘　　要：【目的】微生物在雪茄烟叶发酵过程中起关键作用,为更好地提高雪茄烟品质,需要筛选雪茄优势菌并应用于雪茄烟叶发酵,为此需优化扩大优势菌株的生物量。【方法】对从雪茄烟中筛选出的一株优势菌株ZLX10进行16S测序、生理生化分析以及菌株鉴定,同时通过单因素和正交试验优化培养基成分(碳源、氮源和无机盐)及培养条件(接种量、装液量和种龄),以提高其生物量。【结果】经16S rDNA序列比对,菌株ZLX10与莫海威芽孢杆菌(Bacillus mojavensis,MT043920.1)的序列同源性达到99.86%,该菌株具有很强的多糖和蛋白大分子降解能力,可以鉴定为莫海威芽孢杆菌。经单因素和正交试验得到菌株ZLX10的最优培养基配方为碳源(蔗糖)50 g/L、氮源(酵母提取物)20 g/L、无机盐(MgSO_(4))0.25 g/L,最佳培养条件为接种量1%(V/V)、装液量为250 mL中装30 mL、种龄24 h。在最优培养条件下培养24 h,菌株ZLX10的生物量是优化前的1.98倍。【结论】通过优化条件可以明显提高菌株ZLX10的生物量,为应用于人工接种发酵雪茄烟叶提供基础。【Objective】Microorganisms play a key role in the fermentation process of cigar tobacco leaves.In order to better improve the quality of cigar tobacco leaves,it is necessary to screen the dominant strains of cigars and apply them to the fermentation of cigar tobacco leaves.Therefore,it is necessary to optimize and expand the biomass of the dominant strains.【Method】16S sequencing and physiological and biochemical analysis were carried out on a dominant strain ZLX10 screened from cigars to identify the strain.At the same time,the composition of culture medium(carbon source,nitrogen source and inorganic salt)and culture conditions(inoculation amount,liquid content and seed age)were optimized through single factor and orthogonal test to improve its biomass.【Result】Based on 16S rDNA sequence comparison,the sequence homology between strain ZLX10 and Bacillus mojavensis(MT043920.1)reached 99.86%,and the strain had strong ability to degrade polysaccharide and protein macromolecule,which could be identified as Bacillus mojavenis.Through single factor and orthogonal test,the optimal medium formula of strain ZLX10 was obtained as follows:carbon source(sucrose)50 g/L,nitrogen source(yeast extract)20 g/L,inorganic salt(MgSO_(4))0.25 g/L,and the optimal culture conditions were 1%(V/V)of inoculum,30 mL/250 mL of liquid content,and 24 h of seed age.The biomass of strain ZLX10 was 1.98 times that before optimization when cultured under the optimal conditions for 24 h.【Conclusion】The biomass of strain ZLX10 can be significantly increased by optimizing the conditions,which provides a basis for artificial inoculation of fermented cigar tobacco leaves.

关 键 词：雪茄优势菌株 菌株鉴定 莫海威芽孢杆菌 培养条件优化 生物量 

分 类 号：S572[农业科学—烟草工业]