Single-stranded RNA as primers of terminal deoxynucleotidyl transferase for template-independent DNA polymerization  

作　　者：Houyu Han Jingyu Cui Dianming Zhou Deping Hua Weipan Peng Mengyao Lin Yating Zhang Fangya Li Xiaoqun Gong Jianyu Zhang 

机构地区：[1]School of Life Sciences,Tianjin University and Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology(Tianjin),Tianjin 300072,China [2]School of Pharmaceutical Science and Technology,Tianjin University,Tianjin 300072,China [3]Tianjin Key Laboratory of Pathogenic Microbiology of Infectious Disease,Tianjin Centers for Disease Control and Prevention,Tianjin 300011,China [4]Department of Toxicology,Tianjin Centers for Disease Control and Prevention,Tianjin 300011,China

出　　处：《Chinese Chemical Letters》2023年第2期161-164,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (NSFC, Nos. 21927814 and 21772143 to J.Y. Zhang);the National Science Foundation of Tianjin (Nos. 20YDTPJC00090, 19ZXDBSY00070 and 20YFZCSY00990 to X.Q. Gong)。

摘　　要：Terminal deoxynucleotidyl transferase(Td T) has been characterized as template-independent polymerase using single-stranded DNA(ss DNA) as primers to generate random oligonucleotides. However, the extension performance of Td T to single-stranded RNA(ss RNA) is vague. By systematically comparing and contrasting the performance of Td T-catalyzed ss DNA and ss RNA extension, it is indicated that the catalytic efficiency of ss RNA as primers was about 3 times lower than ss DNA as primers. Collectively, it is believed that understanding the catalytic performance of Td T will help to design the strategy to synthesize chimeric DNA on 3-OH of ss RNA, which becomes invaluable.

关 键 词：Terminal deoxynucleotidyl transferase Single-stranded RNA Single-stranded DNA Template-independent POLYMERIZATION 

分 类 号：Q52[生物学—生物化学]