Near infrared light-induced dynamic modulation of enzymatic activity through polyphenol-functionalized liquid metal nanodroplets  

作　　者：Chaojun Zhang Yang Tang Qin Wang Yunxiang He Xiaoling Wang Sebastian Beyer Junling Guo 

机构地区：[1]BMI Center for Biomass Materials and Nanointerfaces,College of Biomass Science and Engineering,Sichuan University,Chengdu 610065,China [2]School of Pharmacy,Southwest Minzu University,Chengdu 610225,China [3]National Engineering Research Center of Clean Technology in Leather Industry,Sichuan University,Chengdu 610065,China [4]Department of Biomedical Engineering,Chinese University of Hong Kong,Hong Kong 999077,China [5]State Key Laboratory of Polymer Materials Engineering,Sichuan University,Chengdu 610065,China [6]Bioproducts Institute,Department of Chemical and Biological Engineering,University of British Columbia,Vancouver,BC V6T 1Z4,Canada

出　　处：《Chinese Chemical Letters》2023年第2期475-480,共6页中国化学快报（英文版）

基　　金：financial support from the National Talents Program, National Natural Science Foundation of China (Nos. 22178233, 22108181);Talents Program of Sichuan Province, Double First-Class University Plan of Sichuan University, State Key Laboratory of Polymer Materials Engineering (No. sklpme 2020-03-01);the Sichuan Province Postdoctoral Special Funding。

摘　　要：Dynamic manipulation of enzymatic activity is a challenging task for applications in chemical and pharmaceutical industries due to the difficult modification and variable conformation of various enzymes.Here, we report a new strategy for reversible dynamic modulation of enzymatic activity by near-infrared light-induced photothermal conversion based on polyphenol-functionalized liquid metal nanodroplets(LM). The metal-phenolic nanocoating not only provides colloidal stability of LM nanodroplets but also generates nanointerfaces for the assembly of various enzymes on the LM nanodroplets. Upon near infrared(NIR) irradiation, the localized microenvironmental heating through photothermal effect of the LM nanodroplets allows tailoring the enzymatic activity without affecting the bulk temperature. A library of functional enzymes, including proteinase K, glucoamylase, glucose oxidase, and Bst DNA polymerase, is integrated to perform a reversible control and enhanced activities even after five times of cycles, demonstrating great potential in bacterial fermentation, bacteriostasis, and target gene amplification.

关 键 词：Enzymatic activity Photo-modulation Near infrared light Liquid metal nanodroplets Metal-phenolic nanocoating 

分 类 号：TB383.1[一般工业技术—材料科学与工程]