玉屏风散对肺气虚证大鼠JAK1/STAT3通路及炎性反应的影响及相关机制研究  

Effects of Yupingfeng Powder(玉屏风散)on JAK1/STAT3 Pathway and Inflammatory Response in Rats with Lung Qi Deficiency Syndrome and Related Mechanisms

作　　者：吴孝政 黄高 刘杨 陆丽青 蒲水莉 陈云志 WU Xiaozheng;HUANG Gao;LIU Yang;LU Liqing;PU Shuili;CHEN Yunzhi(School of Basic Medicine,Guizhou University of Traditional Chinese Medicine,Guiyang 510025,Guizhou,China;College of Acupuncture-Moxibustion and Tuina,Guizhou University of Traditional Chinese Medicine,Guiyang 510025,Guizhou,China)

机构地区：[1]贵州中医药大学基础医学院,贵州贵阳510025 [2]贵州中医药大学针灸推拿学院,贵州贵阳510025

出　　处：《中华中医药学刊》2023年第3期66-72,I0020,I0021,共9页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(82160861);贵州中医药大学博士启动基金项目(2019-21号)。

摘　　要：目的观察玉屏风散对肺气虚模型大鼠Janus激酶1/信号转导及转录活化因子3(janus kinase 1/signal transducer and activator of transcription 3,JAK1/STAT3)信号通路及其上下游炎性因子的影响,探讨玉屏风散改善肺气虚证的作用机制。方法60只Sprague Dawley(SD)大鼠随机分为空白组,模型组,玉屏风散高、中、低剂量组,阳性对照组,每组10只。除空白组外,其余各组大鼠均采用烟熏加脂多糖气管滴入方法建立肺气虚模型,造模开始灌胃给药,玉屏风散高、中、低剂量组分别予玉屏风散汤液[浓度分别为24、12、6 g/(kg·d)],阳性对照组予地塞米松[0.2 mg/(kg/d)],空白组、模型组给予等体积0.9%生理盐水,连续给药30 d。观察治疗前后各组大鼠症状和体征,肺组织形态学,血清中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)、白细胞介素-8(interleukin-8,IL-8)含量和肺组织中JAK1、STAT3、磷酸化信号转导及转录活化因子3(phosphorylation signal transducer and activator of transcription 3,p-STAT3)、基质金属蛋白酶-9(matrix metalloprotein-9,MMP-9)、组织金属蛋白酶抑制剂1(tissue inhibitor of matrix metalloproteinase 1,TIMP1)蛋白水平的变化。结果与空白组比较,模型组大鼠症状、体征和肺组织损伤状态严重,血清细胞因子TNF-α、IL-6、IL-8显著升高(P<0.01);模型组肺组织中JAK1、STAT3、p-STAT3、MMP-9平均光密度值(Integrated Optical Density,IOD)(P<0.01)和蛋白表达显著升高(P<0.01),TIMP1平均光密度值(P<0.01)和蛋白表达显著降低(P<0.01)。药物治疗后,与模型组比较,玉屏风高剂量组和阳性对照组症状、体征和肺组织损伤状态明显减轻,血清中TNF-α、IL-6、IL-8明显降低(P<0.01),肺组织中JAK1、STAT3、p-STAT3、MMP-9平均光密度值(P<0.01或P<0.05)和蛋白表达明显降低(P<0.01或P<0.05),TIMP1平均光密度值(P<0.01)和蛋白表达明显升高(P<0.01);玉屏风中剂量组症状、体征Objective To observe the effect of Yupingfeng Powder(玉屏风散)on Janus kinase 1/signal transducer and activator of transcription 3(JAK1/STAT3)signaling pathway and its upstream and downstream inflammatory factors in rats with lung Qi deficiency and explore the mechanism of Yupingfeng Powder relieving lung Qi deficiency syndrome.Methods Sixty Sprague Dawley(SD)rats were randomly divided into blank group,model group,high,medium and low dose groups of Yupingfeng Powder,and positive control group,10 rats in each group.Except the blank group,the rats in the other groups were treated with the method of tracheal instillation of smoked lipopolysaccharide to establish the lung Qi deficiency model.After the model was established,the rats were given intragastric administration,and the Yupingfeng Powder high-dose,middle-dose and low-dose groups were given Yupingfeng Powder[24 g/(kg·d),12 g/(kg·d),6 g/(kg·d),respectively].The positive control group was given dexamethasone[0.2 mg/(kg·d)],and the blank group and model group were given an equal volume of 0.9%normal saline for 30 days.Symptoms,lung tissue morphology,tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-8(IL-8)in serum and JAK1,STAT3,phosphorylation signal transducer and activator of transcription 3(p-STAT3),matrix metalloprotein-9(MMP-9),tissue inhibitor of matrix metalloproteinase 1(TIMP1)protein in lung tissue were observed before and after treatment.Results Compared with those in the blank group,the rats in the model group had severe symptoms,signs and lung tissue damage,and the serum cytokines TNF-α,IL-6 and IL-8 were significantly increased(P<0.01).The protein expressions and the integrated optical density(IOD)values of JAK1,STAT3,p-STAT3 and MMP-9 in the lung tissue of the model group were significantly increased(P<0.01)and the IOD value and protein expression of TIMP1 were significantly decreased(P<0.01).After drug treatment,compared with those of the model group,the symptoms,signs and lung tissue damage in the Yupingfeng high-dose grou

关 键 词：玉屏风散 肺气虚 Janus激酶1 信号转导及转录活化因子3 

分 类 号：R285.5[医药卫生—中药学]