慢性自发性荨麻疹患者血清白细胞介素9、血小板活化因子水平与总IgE、病情严重程度和病程的相关性研究  

Correlation of serum interleukin-9 and platelet-activating factor levels with total IgE levels,disease severity and disease course in patients with chronic spontaneous urticaria

作　　者：杨晓静[1] 陈雷刚[1] 王世宁 吴远慧[1] 朱由瑾 安国芝[1] 周向昭[1] 孟昭影[1] Yang Xiaojing;Chen Leigang;Wang Shining;Wu Yuanhui;Zhu Youjin;An Guozhi;Zhou Xiangzhao;Meng Zhaoying(Department of Dermatology,The First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,Hebei,China)

机构地区：[1]河北北方学院附属第一医院皮肤科,张家口075000

出　　处：《中华皮肤科杂志》2023年第4期337-341,共5页Chinese Journal of Dermatology

基　　金：河北省医学科学研究重点课题计划项目(20190896);张家口市级科技计划自筹经费项目(1821063D)。

摘　　要：目的探讨慢性自发性荨麻疹(CSU)患者血清白细胞介素9(IL-9)、血小板活化因子(PAF)水平与血清IgE水平、病情严重程度和病程的相关性。方法收集河北北方学院附属第一医院皮肤科2018年3月至2019年3月收治的60例活动期CSU,按7日荨麻疹活动评分(UAS7)将其分为轻度组、中度组和重度组;同时将经规范抗组胺治疗28 d后进入疾病静止期的患者纳入CSU静止期组。另选同期30例健康体检者为健康对照。采集各组受试者外周静脉血,使用酶联免疫法检测血清IL-9、PAF水平,选用免疫散射比浊法测定血清总IgE水平。分析CSU患者血清IL-9、PAF水平与血清总IgE水平、UAS7评分和病程之间的相关性。多组间比较采用单因素方差分析,组间两两比较采用LSD-t检验,相关性分析采用Pearson检验。结果CSU活动期组男28例,女32例,年龄11~68(34.68±8.62)岁,病程2个月至7年[(1.42±0.41)年]。健康对照组男14例,女16例,年龄10~70(35.06±7.89)岁。轻、中、重度CSU患者分别有12、26、22例,经规范治疗后22例纳入静止期组。CSU活动期组、CSU静止期组、健康对照组血清IL-9水平[(144.34±23.19)、(109.25±20.77)、(107.23±19.23)pg/ml]、PAF水平[(362.45±51.45)、(223.18±32.46)、(221.23±28.38)pg/ml]、总IgE水平[(168.12±32.48)、(24.04±7.04)、(21.76±5.95)IU/ml]差异均有统计学意义(F=38.80、148.38、499.12,均P<0.001),CSU活动期组IL-9、PAF、总IgE水平均显著高于CSU静止期组与健康对照组(P<0.05);CSU静止期组与健康对照组差异均无统计学意义(P>0.05)。血清IL-9水平、PAF水平与血清总IgE水平、UAS7评分均呈正相关(P<0.05),与病程无显著相关性(P>0.05)。结论CSU活动期患者血清IL-9、PAF水平均随病情严重程度的增加而明显升高,且与血清总IgE水平具有密切的相关性。Objective To explore the relationship of serum interleukin(IL)-9 and platelet-activating factor(PAF)levels with serum total immunoglobulin E(IgE)levels,disease severity and disease course in patients with chronic spontaneous urticaria(CSU).Methods A total of 60 patients with active CSU were collected from Department of Dermatology,the First Affiliated Hospital of Hebei North University from March 2018 to March 2019(active CSU group),and divided into mild group,moderate group and severe group according to 7-day urticaria activity score(UAS7).After 28-day standard antihistamine therapy,the patients whose condition became stable were included in the stable CSU group.During the same period,30 health examinees were included in the healthy control group.Peripheral venous blood samples were collected from the subjects in each group,enzyme-linked immunosorbent assay(ELISA)was performed to detect serum levels of IL-9 and PAF,and immunoturbidimetric assay to detect the serum total IgE level.Correlations of serum IL-9 and PAF levels with serum total IgE levels,UAS7 scores and course of disease were analyzed in patients with CSU.One-way analysis of variance was used for comparisons among multiple groups,least significant difference-t test for multiple comparisons,and Pearson correlation analysis for correlation analysis.Results Totally,28 males and 32 females were included in the active CSU group,their age ranged from 11 to 68 years(34.68±8.62 years),and the disease duration ranged from 2 months to 7 years(1.42±0.41 years).In the healthy control group,14 were males and 16 were females,and their age ranged from 10 to 70 years(35.06±7.89 years).According to UAS7,12,26,and 22 patients were diagnosed with mild,moderate and severe CSU respectively,and 22 were included in the stable CSU group after standard treatment.The levels of serum IL-9,PAF and total IgE significantly differed among the active CSU group,stable CSU group and healthy control group(IL-9:144.34±23.19 vs.109.25±20.77 vs.107.23±19.23 pg/ml;PAF:362.45±51.45 v

关 键 词：荨麻疹 白细胞介素9 血小板活化因子 免疫球蛋白E UAS7评分 

分 类 号：R758.24[医药卫生—皮肤病学与性病学]