百合主要有效成分对小鼠B-16细胞黑色素含量、酪氨酸酶活性的影响  

Effects of the main active ingredients from Baihe(Bulbus Lilii)on melanin content and tyrosinase activity in mice B-16 cells

作　　者：彭丽丽[1] 刘朝圣[2] 成雪 陈燕[1] 杨娇娇 李翊澜 PENG Lili;LIU Chaosheng;CHENG Xue;CHEN Yan;YANG Jiaojiao;LI Yulan(Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;The First Hospital of HunanUniversity of Chinese Medicine,Changsha,Hunan 410007,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《湖南中医药大学学报》2023年第4期605-611,共7页Journal of Hunan University of Chinese Medicine

基　　金：湖南省教育厅科学研究项目(17C1231);湖南省中医药科研计划项目(201519);湖南省卫生健康委员会科学研究课题(20201181)。

摘　　要：目的观察百合多糖与百合皂苷对小鼠B-16细胞中细胞活力、酪氨酸酶活性及黑色素含量的影响。方法选用同一传代小鼠B-16细胞并随机分为空白细胞组、空白对照组、熊果苷组、百合多糖组、百合皂苷组,共5组。空白细胞组加入180μL RPMI1640培养液;空白对照组加入180μL RPMI1640培养液和20μL高密度聚乙烯;熊果苷组、百合多糖组、百合皂苷组分别加入不同浓度(0.2、0.4、0.6、0.8 mg/mL)的熊果苷、百合多糖、百合皂苷,作用3 d后,用CCK-8法检测B-16细胞活力,用酪氨酸酶-多巴速率氧化法检测B-16细胞中酪氨酸酶的活性,以MTT法检测小鼠B-16细胞的黑色素含量。结果与空白细胞组比较,0.8 mg/mL熊果苷组细胞活力明显升高(P<0.01),0.4、0.6、0.8 mg/mL百合皂苷组细胞活力均明显降低(P<0.05,P<0.01);各浓度熊果苷、百合多糖组和0.6、0.8 mg/mL百合皂苷组酪氨酸酶活性均明显降低(P<0.01),0.2 mg/mL百合皂苷组酪氨酸酶活性明显升高(P<0.01)。与空白对照组比较,0.8 mg/mL熊果苷组细胞活力明显升高(P<0.01),0.6、0.8 mg/mL百合皂苷组细胞活力均明显降低(P<0.01);各浓度熊果苷、百合多糖组和0.6、0.8 mg/mL百合皂苷组酪氨酸酶活性均明显降低(P<0.01),0.2、0.4 mg/mL百合皂苷组酪氨酸酶活性均明显升高(P<0.01)。与0.2 mg/mL熊果苷组比较,0.8 mg/mL熊果苷组细胞活力、0.2 mg/mL百合皂苷组酪氨酸酶活性均明显升高(P<0.01)。与0.4 mg/mL熊果苷组比较,0.8 mg/mL熊果苷组细胞活力、0.4 mg/mL百合皂苷组酪氨酸酶活性均明显升高(P<0.01)。与0.6 mg/mL熊果苷组比较,0.6 mg/mL百合皂苷组酪氨酸酶活性均明显升高(P<0.01)。与0.4 mg/mL百合皂苷组比较,0.6、0.8 mg/mL百合皂苷组细胞活力均明显降低(P<0.05,P<0.01)。与0.6 mg/mL百合皂苷组比较,0.8 mg/mL百合皂苷组细胞活力明显降低(P<0.01)。在0.2、0.4 mg/mL浓度下,百合皂苷组酪氨酸酶活性均明显高于百合多糖组(P<Objective To investigate the effects of Baihe(Bulbus Lilii)polysaccharides and Baihe(Bulbus Lilii)saponins on cell viability,tyrosinase activity,and melanin content in mice B-16 cells.Methods Mice B-16 cells from the same passage were randomly allocated into 5 groups:blank cell group,blank control group,arbutin group,Baihe(Bulbus Lilii)polysaccharide group,and Baihe(Bulbus Lilii)saponin group.The blank cell group was supplemented with 180μL RPMI1640 culture medium,while the blank control group received 180μL RPMI1640 culture medium and 20μL HDPE.The arbutin,Baihe(Bulbus Lilii)polysaccharide,and Baihe(Bulbus Lilii)saponin groups were treated with varying concentrations(0.2,0.4,0.6 and 0.8 mg/mL)of arbutin,Baihe(Bulbus Lilii)polysaccharide,and Baihe(Bulbus Lilii)saponin,respectively.After 3 d,B-16 cell viability was assessed by CCK-8,tyrosinase activity within B-16 cells was determined via the tyrosinase-dopa oxidation method.Meanwhile,the melanin content in murine B-16 cells was quantified by MTT.Results The cell viability demonstrated a significant increase in the 0.8 mg/mL(P<0.01)and a decrease in the 0.4,0.6 and 0.8 mg/mL Baihe(Bulbus Lilii)saponin groups(P<0.05,P<0.01)compared with the blank cell group.Tyrosinase activity declined obviously in arbutin groups,Baihe(Bulbus Lilii)polysaccharide groups,and Baihe(Bulbus Lilii)saponin group(P<0.01).Tyrosinase activity was notably elevated in the 0.2 mg/mL Baihe(Bulbus Lilii)saponin group(P<0.01).Compared with the blank control group,cell viability was significantly enhanced in the 0.8 mg/mL arbutin group(P<0.01)and markedly reduced in the 0.6 and 0.8 mg/mL Baihe(Bulbus Lilii)saponin groups(P<0.01).Tyrosinase activity displayed a significant decrease in the arbutin and Baihe(Bulbus Lilii)polysaccharide groups and the 0.6 and 0.8 mg/mL Baihe(Bulbus Lilii)saponin groups across all concentrations(P<0.01),while a substantial increase was observed in the 0.2 and 0.4 mg/mL Baihe(Bulbus Lilii)saponin groups(P<0.01).The cell viability in the 0.8 mg/mL arbutin group and th

关 键 词：百合多糖 百合皂苷 熊果苷 B-16细胞 酪氨酸酶 黑色素 

分 类 号：R275.9[医药卫生—中西医结合]