抗稻瘟病和白叶枯病籼稻新种质R156的创制  

Development of new indica rice germplasm with resistance to blast and bacterial blight

作　　者：蔡跃 肖宁[1] 吴云雨[1] 余玲[1] 潘存红[1] 李育红[1] 周长海[1] 季红娟[1] 黄年生[1] 张小祥[1] 左示敏[2] 李爱宏[1,3] CAI Yue;XIAO Ning;WU Yunyu;YU Ling;PAN Cunhong;LI Yuhong;ZHOU Changhai;JI Hongjuan;HUANG Niansheng;ZHANG Xiaoxiang;ZUO Shimin;LI Aihong(Jiangsu Lixiahe Institute of Agricultural Sciences/Yangzhou Comprehensive Test Station of System of Industrial Technology in Rice,Yangzhou 225007,China;Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/College of Agriculture,Yangzhou University,Yangzhou 225009,China;Jiangsu Collaborative Innovation Center for Modern Crop Production,Nanjing 210095,China)

机构地区：[1]江苏里下河地区农业科学研究所/国家水稻产业技术体系扬州试验综合站,江苏扬州225007 [2]扬州大学农学院/植物功能基因组学教育部重点实验室/江苏省作物基因组学和分子育种重点实验室,江苏扬州225009 [3]江苏省现代作物生产协同创新中心,南京210095

出　　处：《扬州大学学报（农业与生命科学版）》2023年第1期21-28,共8页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家现代农业产业技术体系建设专项(CARS-01-65);江苏省现代农业重点研发项目(BE2020318);扬州市现代农业项目(YZ2022043)。

摘　　要：扬稻6号是杂交水稻育种中被广泛应用、具有重要地位的骨干亲本,但其稻瘟病抗性和白叶枯病抗性均普遍偏弱。通过分子标记辅助选择,将抗稻瘟病基因Pigm和Pi33、抗白叶枯病基因Xa21和Xa23导入到扬稻6号,借助重测序技术最大限度地消除每个抗性基因的连锁阻力,构建了高背景回复率近等基因系NIL^(Pigm)、NIL^(Pi33)、NIL^(Xa21)和NIL^(Xa23),表现为稻瘟病或白叶枯病抗性的明显改良。在此基础上,进一步通过基因聚合创制了性状优良、包含所有4个抗性基因的优良株系R156,可作为水稻分子育种的核心种质。Generation of core rice germplasm with resistance to multi-diseases and great breeding potential is increasingly important in current molecular breeding program. Yangdao 6(YD6) has been widely recommended as a very important breeding parent, but it shows weak resistance to both blast and blight diseases. In this study, the elite resistance genes, Pigm and Pi33 showing broad resistance to blast, Xa21 and Xa23 displaying broad resistance to blight, were introduced into YD6 by marker assisted selection. In the aid of re-sequencing technology, the linkage drag of each resistance gene was significantly eliminated, which resulted in the development of four near isogenic lines, NIL^(Pigm), NIL^(Pi33), NIL^(Xa21)and NIL^(Xa23), in YD6 genetic background. The four NILs exhibited high background recovery rate and obvious improvement of resistance to blast and blight. Through gene pyramiding, the elite line, R156, containing all the 4 resistance genes, was developed, which showed excellent performance and could be used as a core germplasm in rice molecular breeding program.

关 键 词：水稻 稻瘟病 白叶枯病 基因聚合 核心种质 

分 类 号：S511[农业科学—作物学]