6种商品化酶抑制-比色法农残速测试剂盒测定5种农药的比较研究  

Comparative study on the determination of five pesticide by six commercial enzyme inhibition colorimetric pesticide residue test kits

作　　者：罗俊霞[1] 刘雪平[2] 申战宾 张刚 张启成[2] 马蕾 张威 赵建波 LUO Junxia;LIU Xueping;SHEN Zhanbin;ZHANG Gang;ZHANG Qicheng;MA Lei;ZHANG Wei;ZHAO Jianbo(Zhengzhou Agricultural Product Quality Testing and Distribution Center,Zhengzhou 450006,China;Zhengzhou Agricultural Technology Extension Centre,Zhengzhou 450008,China;Henan Hengsheng Testing Technology Company Limited,Zhengzhou 451100,China)

机构地区：[1]郑州市农产品质量检测流通中心,郑州450006 [2]郑州市农业技术推广中心,郑州450008 [3]河南恒晟检测技术有限公司,郑州451100

出　　处：《扬州大学学报（农业与生命科学版）》2023年第1期124-131,共8页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：郑州市农产品质量检测中心建设项目(豫发改农经[2011]1778号);郑州市检测检验体系建设项目(郑农检[2012]12号);郑州市农产品质量安全追溯体系建设项目(郑发改农经[2013]126号)。

摘　　要：使用6种商品化酶抑制-比色法农残速测试剂盒在5个浓度水平下测定乐果等5种农药的抑制率,绘制抑制率对农药浓度的效应曲线图,用抑制率和农药浓度的对数进行线性拟合,求出决定系数、IC_(50)和IC_(10),根据IC_(10)以试剂盒对乐果等5种农药的敏感性进行比较研究。结果表明:6种试剂盒对乐果和甲基异柳磷均不敏感,对其他3种农药的敏感性也各不相同,就所测定的5种农药而言,试剂盒C较优。综合而言,为适应市场监管和试剂盒检测方便,建议生产商在生产试剂盒时,对所使用的酶和底物进行测定,必要时将几种不同来源、性质相近的酶按一定的比例进行混合,以使试剂盒敏感的农药更多,从而减少假阴性样品。The inhibition rates of dimethoate and other five pesticides were determined by six commercial enzyme inhibition test kits at five concentration levels, and the broken line graph of inhibition rate to pesticide concentration was drawn. The logarithm of inhibition rate and pesticide concentration was used for linear fitting, and the correlation coefficient, IC_(50)and IC_(10)were calculated. The sensitivity of various kits to five pesticides was compared according to IC_(10). The results showed that the six kits were not sensitive to dimethoate and isofenphos methyl, and the sensitivity of the six kits to the other three pesticides were also different. Due to the different sensitivity of commercial kits to all kinds of pesticides and the variety of pesticides, it is difficult to screen kits in daily supervision. In order to adapt to market supervision and the adaptability of kits, it was suggested that manufacturers should determine the enzymes and substrates used in the production of kits, and if necessary, compare the properties of several different sources. In order to make the kit more sensitive to pesticides and reduce false negative samples.

关 键 词：检测试剂盒 农药 半抑制浓度 检出限 敏感性 线性范围 

分 类 号：X592[环境科学与工程—环境工程]