腮腺炎减毒活疫苗(F-基因型)免疫血清中和抗体效价与特异性IgG浓度的比较分析  

Comparative analysis of neutralizing antibody titer and specific IgG concentration in sera immunized with mumps attenuated live vaccine(F-genotype)

作　　者：赵恒[1] 廖芸[1] 将国润 李丹丹 张莹[1] 范胜涛[1] 于丽 李琦涵[1] ZHAO Heng;LIAO Yun;JIANG Guorun;LI Dandan;ZHANG Ying;FAN Shengtao;YU Li;LI Qihan(Institute of Medical Biology,Chinese Academy of Medical Sciences,Peking Union Medical College,Kunming 650118,Yunnan Province,China)

机构地区：[1]中国医学科学院北京协和医学院,医学生物学研究所,云南昆明650118

出　　处：《中国生物制品学杂志》2023年第3期263-268,共6页Chinese Journal of Biologicals

基　　金：云南省科技重大专项(2019ZF00101)。

摘　　要：目的 对腮腺炎减毒活疫苗(F-基因型)免疫血清中和抗体效价及特异性IgG浓度进行比较分析,评价二者作为相互验证指标的可能性。方法 选取194份人免疫血清,包括疫苗组98份[腮腺炎减毒活疫苗(F-基因型),免疫前后各49份]和安慰剂组96份(不含腮腺炎病毒,其他成分与疫苗组相同,免疫前后各48份),采用ELISA法和中和试验分别检测血清腮腺炎病毒特异性IgG浓度和中和抗体效价,并对两个指标的检测结果进行比较。以中和抗体效价为纵坐标,IgG浓度为横坐标绘制散点图,通过逻辑回归分析二者的相关性。以中和抗体效价为标准采用ROC曲线对各组特异性IgG浓度进行分析,评价以特异性IgG浓度判定中和抗体阳转的可靠性。结果 安慰剂组免疫前血清特异性IgG浓度(t=-0.977,P> 0.05)及中和抗体效价与免疫后比较,差异均无统计学意义(Z=-1.405,P> 0.05),疫苗组免疫后血清特异性IgG浓度(t=-9.959,P <0.001)及中和抗体效价(Z=-5.696,P <0.001)均明显高于免疫前。安慰剂组免疫前后及疫苗组免疫前IgG阳转率均明显高于相应中和抗体阳转率(χ^(2)分别为27.927、29.777、17.563,P均<0.001),疫苗组免疫后IgG阳转率与中和抗体阳转率差异无统计学意义(χ^(2)=2.346,P> 0.05)。各组特异性IgG浓度与中和抗体效价间具有显著相关性(r=0.321 0~0.620 1,P均<0.05)。疫苗组免疫后的ROC曲线下面积最大(0.961),特异性(1.00)和灵敏度(0.93)较高。结论 腮腺炎减毒活疫苗(F-基因型)免疫血清中和抗体效价与特异性IgG浓度具有良好的相关性,二者可作为相互验证的指标。Objective To compare and analyze neutralizing antibody titer and specific IgG concentration in the sera immunized with F-genotype mumps attenuated live vaccine,so as to evaluate the possibility of using them as mutual verification indexes.Methods A total of 194 serum samples were randomly selected,including 98 in vaccine group inoculated with F-genotype mumps attenuated live vaccine(49 before immunization and 49 after immunization)and 96 in placebo group(48 before immunization and 48 after immunization),which were detected for the concentration of mumps virus specific IgG and the titer of neutralizing antibody by ELISA and neutralization test respectively,and the detection results were compared.With neutralizing antibody titer as ordinate and IgG concentration as abscissa,the scatter plot was drawn,and the correlation between them was analyzed by logistic regression.With neutralizing antibody titer as standard,the specific IgG concentration of each group was analyzed by ROC curve,which was evaluated to judge the reliability of neutralizing antibody positive conversion.Results There was no significant difference in the concentration of specific IgG(t =-0.977,P>0.05)and the titer of neutralizing antibody(Z =-1.405,P>0.05)in the serum of the placebo group before and after immunization.However,in the vaccine group,the concentration of specific IgG(t =-9.959,P<0.001)and the titer of neutralizing antibody(Z =-5.696,P<0.001)in the serum after immunization were both significantly higher than those before immunization;The positive conversion rates of specific IgG before and after immunization in the placebo group and before immunization in the vaccine group were significantly higher than those of neutralizing antibody(χ^(2)= 27.927,29.777 and 17.563respectively,each P<0.001),while no significant difference was observed between the positive conversion rates of the two after immunization in the vaccine group(χ^(2)= 2.346,P>0.05).There was a significant correlation between specific IgG concentration and neutralizing antibo

关 键 词：腮腺炎减毒活疫苗 中和抗体 IGG 相关性 ROC曲线 

分 类 号：R373.2[医药卫生—病原生物学]