丁香油对秀丽隐杆线虫的毒杀作用及其机理  

Toxicity and mechanism of clove oil on Caenorhabditis elegans

作　　者：段昊沅 陈华铮 王侨 朱凯[1] DUAN Haoyuan;CHEN Huazheng;WANG Qiao;ZHU Kai(College of Chemical Engineering,Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,Nanjing Forestry University,Nanjing 210037,China)

机构地区：[1]南京林业大学化学工程学院,江苏省林业资源高效加工利用协同创新中心,南京210037

出　　处：《林业工程学报》2023年第2期95-100,共6页Journal of Forestry Engineering

基　　金：国家重点研发计划(2018YFD0600405)。

摘　　要：丁香油中含有多种活性成分,具有杀虫、抑菌、抗氧化等功效,且不存在残留和耐药性问题,为了解决农业方面化学合成药剂大量使用所带来的病虫害抗药性、环境污染以及对人类健康安全等问题,以秀丽隐杆线虫作为模式生物,研究丁香油对线虫的毒杀作用。实验结果表明,丁香油和丁香酚对秀丽隐杆线虫的半致死质量浓度(IC50)分别为55.74和29.22 mg/L;其能抑制线虫体内过氧化氢酶、谷胱甘肽酶和超氧化物歧化酶活性,破坏氧化和抗氧化的动态平衡,减少线虫的产卵量。通过RNA-seq序列分析,从基因层面上解读丁香油和丁香酚对线虫的作用,发现作用于线虫代谢功能的基因受到调控,如E01G6.1、cht-1、C40H1.8、lipl-5、fat-2、txt-8、fat-4、acox-1.2、dagl-2和pigw-1等;对hsp-70和F44E5.5等基因的调控,使得线虫体内蛋白质,尤其是热休克蛋白的产生和工作受到影响。丁香油对秀丽隐杆线虫有较强的毒性,且能进入线虫体内对其造成破坏,从根源上减少线虫的危害。由此可知,丁香油可与传统化学药剂复配使用,从而减少传统化学药剂用量,作为绿色杀虫剂在农业领域的应用中具有极大的开发价值。Plant essential oils are natural and renewable resources with a wide variety. Clove oil contains a variety of active ingredients, which have insecticidal, bacteriostatic, antioxidant and other effects, and there are no residue and drug resistance problems. Therefore, in order to solve the problems of pest resistance, environmental pollution and human health and safety caused by the extensive use of chemical synthetic agents in agriculture, the model organism Caenorhabditis elegans was used to investigate the effectivity of the clove oil on killing C. elegans. Utilization of the concentration mass of 10-1 000 mg/L clove oil, the mortality of nematodes increased from 20.62% to 98.97%. The mortality of nematodes increased from 28.87% to 100% at the mass concentration of 10-1 000 mg/L eugenol. The IC50values of the clove oil and eugenol against C. elegans were 55.74 and 29.22 mg/L, respectively. Effects of the clove oil on C. elegans included inhibited activities of catalase, glutathione and superoxide dismutase in nematodes, destruction of the dynamic balance of oxidation and anti-oxidation, which reduced the fecundity by C. elegans. The mortality of nematodes treated with the 10 mg/L clove oil was 46.67%, which was 1.40 times that of the blank control. The mortality of nematode treated with eugenol of the same concentration was 52.50%, reaching 1.58 times. The mortality rate of nematodes treated with the 100 mg/L clove oil and eugenol was 56.41% and 60.00%, which were 1.69 and 1.80 times higher than that of the blank control, respectively. The RNA-seq analysis demonstrated that the clove oil and eugenol could affect nematodes at the genetic level, as genes that are known to be involved in nematode metabolic functions, such as E01G6.1, cht-1, C40H1.8, lipl-5, fat-2, txt-8, fat-4, acox-1.2, dagl-2 and pigw-1, are affected. The regulation of the eugenol and clove oil on hsp-70 and F44E5.5 impacted the production and function of proteins in nematode, especially those of heat shock proteins. The results showed that the

关 键 词：秀丽隐杆线虫 丁香油 丁香酚 毒杀 酶活 

分 类 号：TQ651[化学工程—精细化工]