海南岛火焰兰SSR标记开发、遗传多样性与群体结构分析  

SSR Marker Development,Genetic Diversity and Population Structure Analyses of Renanthera coccinea in Hainan

作　　者：赵慧琪 李栋梁[1,2] 赵慧琳 吴芳芳 黄赛 张永豪[1,2] 张浪 杨珺 任军方[1,2] Zhao Huiqi;Li Dongliang;Zhao Huilin;Wu Fangfang;Huang Sai;Zhang Yonghao;Zhang Lang;Yang Jun;Ren Junfang(Sanya Institute,Hainan Academy of Agricultural Sciences,Sanya,572025;National Germplasm Resource Chengmai Observation and Experiment Station,The Key Laboratory of Tropic Special Economic Plant Innovation and Utilization,Institute of Tropical Horticulture Research,Hainan Academy of Agricultural Sciences,Haikou,571100;Plant Protection and Quarantine Center of Shanxi Province,Taiyuan,030001;School of Horticulture,Hainan University,Haikou,571100)

机构地区：[1]海南省农业科学院三亚研究院,三亚572025 [2]海南省农业科学院热带园艺研究所,海南省特种经济植物种质资源创新利用重点实验室,国家种质资源澄迈观测实验站,海口571100 [3]山西省植物保护植物检疫中心,太原030001 [4]海南大学园艺学院,海口571100

出　　处：《分子植物育种》2023年第7期2300-2310,共11页Molecular Plant Breeding

基　　金：海南省自然科学基金项目(321QN361);海南省重点研发项目(ZDYF2022XDNY254);海南省技术创新专项(jscx202010);2022年乡村振兴及百镇千村补助资金项目(2022-04-12)共同资助。

摘　　要：为了开发火焰兰(Renanthera coccinea)的SSR引物,进而开展海南岛火焰兰的种质鉴定、遗传多样性和群体结构分析。本研究采用三代测序技术首先获得火焰兰参考转录组,然后开发了火焰兰的SSR引物,最后基于SSR引物利用分子变异分析(AMOVA)、非加权组平均法(UPGMA)聚类、主坐标(PCoA)与群体结构分析等方法对来自海南岛不同地理分布的396份火焰兰资源进行研究。结果表明,所开发的17对三核苷酸多态性SSR引物能够有效地鉴别所收集的396份火焰兰种质。AMOVA分析显示,火焰兰群体间呈现极高程度的遗传分化,并且群体内的遗传变异高于群体间的遗传变异。群体结构分析显示,来自不同地理分布的海南岛火焰兰存在3个不同的基因库,而且绝大多数群体是独立进化的,只有少数群体存在遗传混合,这一结果与UPGMA和PCoA分析的结果基本一致。综上可知,本研究新开发的17对SSR引物有效鉴定了396份海南岛火焰兰种质,揭示了其遗传多样性和群体遗传结构,对火焰兰的种质资源保护和育种计划具有非常重要的参考价值。In order to develop SSR primers for Renanthera coccinea,and then to carry out its germplasm identification,genetic diversity and population structure analyses.Using third generation sequencing technology,we firstly obtained a reference transcriptome of R.coccinea,based on which SSR primers were developed.By using these primers,we performed a series of analyses on 396 germplasms collected from various areas in Hainan Island,including AMOVA,UPGMA,PCoA and population structure analyses.The results showed that the newly developed 17 trinucleotide polymorphic SSR primer pairs could effectively distinguish the 396 R.coccinea germplasms.AMOVA revealed high genetic differentiation among populations.Meanwhile,the genetic variation within populations was higher than that among populations.Population structure analysis showed that three different gene pools were present in R.coccinea germplasms from different geographical distribution in Hainan Island and most of the populations evolved independently with genetic admixture occurring in only a few populations.These findings were basically consistent with the results of UPGMA and PCoA analyses.Altogether,our study developed 17 new SSR primer pairs,which could effectively identify the 396 R.coccinea germplasms in Hainan Island.Our findings on their genetic diversity and population genetic structure provide helpful insights regarding the germplasm conservation and breeding of R.coccinea.

关 键 词：火焰兰 SSR标记 遗传多样性 群体结构 

分 类 号：S682.31[农业科学—观赏园艺]