微流控芯片技术研究梯度变化的剪切力诱导的血小板粘附、聚集效应  

Study on Platelet Adhesion and Aggregation Induced by Gradient Shear Stress Using Microfluidic Chip Technology

作　　者：马海东[1] 何翠[2] 邓素容[2] 张婷婷[2] 李远[3] 张天聪 MA Hai-Dong;HE Cui;DENG Su-Rong;ZHANG Ting-Ting;LI Yuan;ZHANG Tian-Cong(Pharmacy Department,Yongchuan Hospital of Chongqing Medical University,Chongqing 402160,China;Department of Blood Transfusion,Yongchuan Hospital of Chongqing Medical University,Chongqing 402160,China;Central Laboratory,Yongchuan Hospital of Chongqing Medical University,Chongqing 402160,China)

机构地区：[1]重庆医科大学附属永川医院药学部,重庆402160 [2]重庆医科大学附属永川医院输血科,重庆402160 [3]重庆医科大学附属永川医院中心实验室,重庆402160

出　　处：《中国实验血液学杂志》2023年第2期495-502,共8页Journal of Experimental Hematology

基　　金：重庆市教委科技研究计划项目(kjqn202100442);重庆医科大学附属永川医院科研项目(YJJC202106、yjlc201915、YJLC201707)。

摘　　要：目的:应用微流控芯片分析技术研究梯度变化的剪切力对血小板聚集的影响。方法:应用微流控芯片模拟80%固定狭窄微通道,微通道狭窄模型利用三维建模软件sollidwork自带的有限元分析模块进行流体动力学行为分析。应用微流控芯片分析不同疾病患者血液的血小板粘附和聚集行为,以及采用流式细胞术检测血小板活化标志物CD62p的表达。同时使用抗血小板药物阿司匹林、替罗非班、原儿茶酸、CD42b处理血液,并通过荧光显微镜实时观察血小板粘附和聚集行为。结果:微流控芯片狭窄模型产生的梯度变化的流体剪切率能够诱导血小板聚集,且在一定剪切率范围内血小板粘附聚集程度随着剪切率的增加而增加。动脉血栓性疾病患者的血小板聚集效应明显高于正常对照组(P<0.05),骨髓增生异常患者血小板聚集效应低于正常对照组(P<0.05)。结论:在剪切力存在环境下微流控芯片分析技术能够准确分析评估各种血栓性疾病的血小板黏附聚集效应,有助于临床血栓类疾病的辅助诊断。Objective:To study the effect of gradient shear stress on platelet aggregation by microfluidic chip Technology.Methods:Microfluidic chip was used to simulate 80%fixed stenotic microchannel,and the hydrodynamic behavior of the stenotic microchannel model was analyzed by the finite element analysis module of sollidwork software.Microfluidic chip was used to analyze the adhesion and aggregation behavior of platelets in patients with different diseases,and flow cytometry was used to detect expression of the platelet activation marker CD62p.Aspirin,Tirofiban and protocatechuic acid were used to treat the blood,and the adhesion and aggregation of platelets were observed by fluorescence microscope.Results:The gradient fluid shear rate produced by the stenosis model of microfluidic chip could induce platelet aggregation,and the degree of platelet adhesion and aggregation increased with the increase of shear rate within a certain range of shear rate.The effect of platelet aggregation in patients with arterial thrombotic diseases were significantly higher than normal group(P<0.05),and the effect of platelet aggregation in patients with myelodysplastic disease was lower than normal group(P<0.05).Conclusion:The microfluidic chip analysis technology can accurately analyze and evaluate the platelet adhesion and aggregation effects of various thrombotic diseases unde the environment of the shear rate,and is helpful for auxiliary diagnosis of clinical thrombotic diseases.

关 键 词：微流控芯片 剪切率 梯度变化 血小板粘附聚集 

分 类 号：R55[医药卫生—血液循环系统疾病]