LED混合光对螺旋藻生长及藻蓝蛋白积累的影响  

Effects of LED mixed light on growth and phycocyanin accumulation of Spirulina

作　　者：谢佳文 徐佩伦 韩佩[1] 钱军[1] 张佩东 王邦 郭凌玥 周文广[1] XIE Jiawen;XU Peilun;HAN Pei;QIAN Jun;ZHANG Peidong;WANG Bang;GUO Lingyue;ZHOU Wenguang(Key Laboratory of Poyang Lake Environment and Resources Utilization,Ministry of Education,School of Resources and Environment,Nanchang University,Nanchang 330031,China)

机构地区：[1]南昌大学资源与环境学院鄱阳湖环境与资源利用教育部重点实验室,南昌330031

出　　处：《生物学杂志》2023年第2期58-64,共7页Journal of Biology

基　　金：江西省重点研发项目(20214BBG74004);国家自然基金项目(31960734)。

摘　　要：为探究不同光质对螺旋藻生长及藻蓝蛋白积累的影响规律,以发光二极管(LED)为光源,在白光(W)、蓝光(B)、红光(R)单色光和白、蓝、红9种不同光强比例组合条件下培养螺旋藻,测定螺旋藻的生物量、藻蓝蛋白的含量及产量。结果表明:红光对螺旋藻的生长速度有促进作用,蓝光有利于提高螺旋藻藻蓝蛋白含量及纯度;B+R(75%+25%)组OD 560比蓝光提高了154%,干重提高了157%,藻蓝蛋白含量比红光提高了43%,藻蓝蛋白纯度比红光提高了22%;B+W(75%+25%)组OD 560比蓝光提高了107%,干重提高了119%,藻蓝蛋白含量、纯度与蓝光单色光接近;W+R(50%+50%)组藻蓝蛋白产量最高,为42 mg/(L·d)。To explore the effects of different light equality on growth and phycocyanin accumulation of Spirulina,the algal cells were cultured in three monochromatic lights(i.e.white(W),blue(B),red(R)light)and their nine mixed light(mixed light intensity ratios)using light emitting diode(LED)as light source.Simultaneously,the algal yield,phycocyanin content and productivity were measured during the experimental period.The results showed that the red group promoted the growth rate of Spirulina,while the blue group contributed to improve both of phycocyanin content and purity in algal cells.Algal yield as optical density(OD)and dry weight in B+R(75%+25%)group increased by 154%and 157%compared with that in the blue group,whereas phycocyanin content and purity increased by 43%and 22%compared with that in the red group,respectively.OD value and dry weight in B+W(75%+25%)group were improved by 107%and 119%,respectively,compared to that in the blue group,but each of phycocyanin content and purity was almost same as that in blue group.The phycocyanin productivity in W+R(50%+50%)group was highest,42 mg/(L·d),among the tested groups.

关 键 词：LED 光质 藻蓝蛋白 生物量 

分 类 号：Q945[生物学—植物学] Q949.22