冬眠期与非冬眠期达乌尔黄鼠小肠AQP1和AQP3基因表达及消化酶活力的比较  

Comparison on genes expression of AQP1 and AQP3 and activity of digestive enzymes in small intestine between hibernating and non-hibernating Daurian ground squirrels

作　　者：钟秋梅 王建礼[1] ZHONG Qiumei;WANG Jianli(College of Biological Sciences and Engineering,North Minzu University,Yinchuan 750021,China)

机构地区：[1]北方民族大学生物科学与工程学院,银川750021

出　　处：《生物学杂志》2023年第2期86-90,共5页Journal of Biology

基　　金：宁夏自然科学基金项目(2020AAC03244);国家自然科学基金项目(32160245)。

摘　　要：达乌尔黄鼠(Spermophilus dauricus)是一种贮脂类冬眠动物,冬眠期间不摄入食物和水分,在此期间其肠道水通道蛋白(aquaporin,AQP)基因表达和消化酶活力与非冬眠期相比有何不同还不清楚。通过检测夏季活动期(7月)、冬眠期(12月)和春季出眠期(3月)达乌尔黄鼠回肠AQP1和AQP3基因的表达及蛋白酶、麦芽糖酶、蔗糖酶和脂肪酶的活力,探讨不同时期小肠生理生化水平的适应性。结果发现,冬眠期达乌尔黄鼠AQP1基因表达高于夏季活动期和春季出眠期,AQP3基因表达低于活动期和出眠期。冬眠期和出眠期的蛋白酶活力低于活动期;冬眠期麦芽糖酶和蔗糖酶活力低于出眠期;不同时期脂肪酶活力无显著变化。结果表明,达乌尔黄鼠可改变小肠AQP基因表达和部分消化酶的活力调节水分平衡和营养,以适应其冬眠习性和能量需求的变化。The Daurian ground squirrel(Spermophilus dauricus)is a lipid storage hibernating animal,as a result,it does not eat any food and drink water during hibernation.However,it is not well known what the differences are in genes expression of Aquaporin(AQP)and activity of digestive enzymes between hibernating and non-hibernating periods.To investigate the adaptive response of small intestine at physiological and biochemical levels in hibernating rodents,the genes expression of AQP1 and AQP3 and the activity of digestive enzymes(protease,sucrase,maltase and lipase)in the ileum of Daurian ground squirrels were examined during active(summer,July),hibernating(winter,December)and arousal(spring,March)periods.The results indicated the expression level of AQP1 gene in hibernating period was higher than that in active and arousal periods,while the expression level of AQP3 gene in hibernating period was lower than that in active and arousal periods.Total protease activity was higher in active period than that in hibernating and arousal periods.The activity of maltase and sucrase in hibernating period was lower than that in arousal period.The activity of lipase was relatively stable in these three periods.These results indicate that the ground squirrel regulates the water balance and nutrient by changing the AQP genes expression and the some enzymes activity of small intestine,which could acclimate to its hibernation habit and variations in energy demands.

关 键 词：达乌尔黄鼠 水通道蛋白 消化酶 冬眠 小肠 

分 类 号：Q958[生物学—动物学] Q786