江苏省笼养雪貂隐孢子虫感染及分子鉴定  

Prevalence and molecular characterization of Cryptosporidium in captive-bred Mustela putorius furo in Jiangsu Province

作　　者：张金洋 周永华[2] 郭杰 李加佳 吴严严 周正海 朱海云 罗昕雨 陈东钱 李乔乔 刘欣超 李文超[1] ZHANG Jinyang;ZHOU Yonghua;GUO Jie;LI Jiajia;WU Yanyan;ZHOU Zhenghai;ZHU Haiyun;LUO Xinyu;CHEN Dongqian;LI Qiaoqiao;LIU Xinchao;LI Wenchao(Anhui Province Key Laboratory of Animal Nutritional Regulation and Health,College of Animal Science,Anhui Science and Tech-nology University,Fengyang,Anhui 233100,China;Jiangsu Institute of Parasitic Diseases,Key Laboratory of National Health Commission Parasitic Disease Control and Prevention,China;Animal Husbandry Development Center of Lu’an City,China)

机构地区：[1]安徽科技学院动物科学学院动物营养调控与健康安徽省重点实验室,安徽凤阳233100 [2]江苏省血吸虫病防治研究所、国家卫生健康委员会寄生虫病预防与控制技术重点实验室 [3]安徽省六安市畜牧业发展中心

出　　处：《中国血吸虫病防治杂志》2023年第1期73-77,共5页Chinese Journal of Schistosomiasis Control

基　　金：安徽省自然科学基金(1808085MC84,1908085QC116);安徽科技学院校级稳定人才项目(dkwd201702);省级大学生创新创业项目(S202110879166,S202210879196)。

摘　　要：目的了解江苏省某养殖场笼养雪貂隐孢子虫感染率及分子特性。方法2017年5月从江苏省某雪貂养殖场共采集290份新鲜雪貂粪便样本,采用基于隐孢子虫核糖体小亚基rRNA(SSU rRNA)基因的巢式PCR方法检测所有粪便样本,对获得的阳性样本扩增actin、cowp、gp60基因,扩增产物测序,以鉴定隐孢子虫虫种和基因型。结果基于隐孢子虫SSU rRNA基因的巢式PCR共鉴定出18份隐孢子虫阳性样本,隐孢子虫检出率为6.2%。SSU rRNA、actin基因和cowp基因序列分析及进化树分析均显示,笼养雪貂感染的隐孢子虫为隐孢子虫雪貂基因型(Cryptosporidium sp.ferret genotype)。18份隐孢子虫阳性样本中,10份样本扩增出gp60基因。结论江苏省笼养雪貂中隐孢子虫感染比较普遍,隐孢子虫雪貂基因型是检测到的唯一基因型。Objective To investigate the prevalence and molecular features of Cryptosporidium in captive-bred Mustela putorius furo in Jiangsu Province.Methods A total of 290 fresh stool samples were collected from a ferret farm in Jiangsu Province on May 2017,and the small subunit rRNA(SSU rRNA)gene of Cryptosporidium was amplified in stool samples using nested PCR assay.The actin,cowp and gp60 genes were amplified in positive samples and sequenced to characterize Cryptosporidium species/genotypes.Results A total of 18 stool samples were tested positive for Cryptosporidium SSU rRNA gene,with a detection rate of6.2%.Sequence and phylogenetic analyses of SSU rRNA,actin and cowp genes characterized Cryptosporidium isolated from captive-bred ferrets as Cryptosporidium sp.ferret genotype.In addition,gp60 gene was amplified in 10 out of 18 stool samples tested positive for Cryptosporidium.Conclusions Cryptosporidium is widely prevalent in captive-bred ferrets in Jiangsu Province,and Cryptosporidium sp.ferret genotype is the only Cryptosporidium genotype in ferrets.

关 键 词：隐孢子虫 核糖体小亚基rRNA 分子鉴定 雪貂 江苏省 

分 类 号：R382.3[医药卫生—医学寄生虫学]