五倍子水提液与抗生素联合对耐药大肠杆菌的抗菌作用研究  

Study on Antibacterial Effect of Galla chinensis Aqueous Extract Combined with Antibiotics on Drug-resistant Escherichia coli

作　　者：李云超 魏思敏 田启明 李金贵[1] LI Yunchao;WEI Simin;TIAN Qiming;LI Jingui(College of Veterinary Medicine,Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou University,Jiangsu Yangzhou 225009,China)

机构地区：[1]扬州大学兽医学院,江苏省高校动物重要疫病与人畜共患病防控协同中心,江苏扬州225009

出　　处：《饲料工业》2023年第7期99-105,共7页Feed Industry

基　　金：国家自然科学基金资助项目[32072911];江苏省高校优势学科建设工程项目[PAPD20190601]。

摘　　要：为探讨五倍子水提液(Galla chinensis extract,GCE)与临床常用抗生素联用对耐药大肠杆菌(E.coli)的体内外抑制效果,试验采用微量肉汤稀释法检测GCE和不同抗生素联用对临床分离E.coli的最小抑菌浓度(minimal inhibitory concentration,MIC);采用棋盘稀释法进行联合药敏试验,计算GCE和抗生素联用时的分级抑菌浓度(fractional inhibitory concentration,FIC),判定药物间的相互作用;采用E.coli感染模型小鼠进一步验证GCE与抗生素联合应用的体内效果。结果表明:GCE对E.coli标准菌株和多重耐药菌株均有明显的抑制作用,MIC范围为4~8 mg/mL;GCE与β-内酰胺类抗生素联合的FIC小于0.5,表现为协同抑菌作用;在E.coli耐药菌株(MC02)动物感染模型中,与单独用药相比,GCE与阿莫西林联合可明显提高感染小鼠的存活率和增重,并减轻肠道病变。提示GCE可增强β-内酰胺类抗生素对耐药菌的抑制作用。In order to investigate the effect of Galla chinensis aqueous extract(GCE)combined with clini⁃cally used antibiotics on drug-resistant Escherichia coli(E.coli)in vivo and in vitro,the minimal inhibito⁃ry concentration(MIC)of GCE and different antibiotics on clinically isolated E.coli was detected by mi⁃cro broth dilution method.Then,the chessboard dilution method was used to carry out the combined drug sensitivity test,and the fractional inhibitory concentrations(FIC)of GCE and antibiotics were calcu⁃lated to determine the interactive effect of them.Further more,the E.coli infected mice model was used to evaluate the in vivo synergistic effect of GCE and antibiotics.The results showed that GCE had obvi⁃ous inhibitory effect on the standard strain and multidrug resistant E.coli strains with the MIC range of 4 to 8 mg/mL.The FIC of GCE combined withβ-lactam antibiotics is less than 0.5,which showed syner⁃gistic antibacterial effect.In the animal infection model induced by a drug-resistant E.coli strain(MC02),compared with single drug,the combination of GCE and amoxicillin had obvious advantages in reducing death rate and weight gain,along with alleviating intestinal pathology.These indicate GCE could en⁃hance the inhibitory effect ofβ-lactam antibiotics on drug-resistant bacteria.

关 键 词：五倍子提取物 抗生素 协同 耐药大肠杆菌 抗菌作用 

分 类 号：S816.7[农业科学—饲料科学]