杏香兔耳风化学成分研究  

Study on chemical constituents of Ainsliaea fragrans

作　　者：徐贞权 何明珍[1] 姚闽 王昭君 欧阳辉[1] 李志峰[1] 杨世林[1,2] 李军茂 冯育林 XU Zhen-quan;HE Ming-zhen;YAO Min;WANG Zhao-jun;OUYANG Hui;LI Zhi-feng;YANG Shi-lin;LI Jun-mao;FENG Yu-lin(National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal.Medicine,Jiangxi University of Traditional Chinese Medicine,Nanchang 330006,China;State Key Laboratory of Innovative Medicine and High Efficiency and Energy Saving Pharmaceutical Equipment,Nanchang 330006,China;Jiangxi Institute for Drug Control,Nanchang 330029,China)

机构地区：[1]江西中医药大学,中药固体制剂制造技术国家工程研究中心,江西南昌330006 [2]创新药物与高效节能降耗制药设备国家重点实验室,江西南昌330006 [3]江西省药品检验检测研究院,江西南昌330029

出　　处：《中草药》2023年第6期1728-1735,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(82173945);国家自然科学基金资助项目(81760702);国家自然科学基金资助项目(81560636);江西省教育厅科学技术研究项目(GJJ211261);江西省食品监督管理局科研项目(2020JS10);江西省卫生健康委员会科技计划项目(202211429);江西省中医药管理局科技计划项目(2022A366);江西中医药大学创新基金项目(JZYC22S60);江西中医药大学校级科技创新团队发展计划(CXTD22001)。

摘　　要：目的研究兔耳风属植物杏香兔耳风Ainsliaea fragrans的化学成分。方法采用溶剂萃取、大孔吸附树脂、硅胶、ODS柱色谱以及HPLC等技术进行化学成分分离纯化,运用核磁共振等波谱数据分析鉴定化合物结构。结果从杏香兔耳风乙醇提取物中分离得到20个化合物,分别鉴定为3β-O-β-D-glucopyranosyl-8α-hydroxy-11α,13-dihydrozaluzanin C(1)、岩白菜素(2)、glucozaluzanin C(3)、芹菜素-7-O-β-D-葡萄糖醛酸甲酯(4)、costunolide-15-β-D-glucopyranoside(5)、芹菜素-7-O-β-D-葡萄糖醛酸苷(6)、木犀草素-7-O-β-D-葡萄糖醛酸苷(7)、芹菜素-6,8-二-C-葡萄糖苷(8)、绿原酸(9)、1,3-O-二咖啡酰奎宁酸(10)、3-O-反式对羟基香豆酰奎宁酸(11)、木犀草素-7-O-β-D-葡萄糖苷(12)、木犀草素(13)、原儿茶酸(14)、5-O-顺式对羟基香豆酰奎宁酸(15)、1-O-咖啡酰奎宁酸(16)、隐绿原酸(17)、5-O-反式对羟基香豆酰奎宁酸(18)、3,5-O-二咖啡酰奎宁酸(19)、2′-O-E-caffeoyl-8α-hydroxy-11α,13-dihydro-3β-O-β-D-glucozaluzaninC(20)。结论化合物2、4~8、11、15~16、18为首次从兔耳风属植物中分离得到。Objective To investigate chemical constitutes of Ainsliaea fragrans.Methods The compounds were isolated and purified by solvent extraction,macroporous adsorbent resin,silica gel column chromatography,ODS and semi-preparative HPLC.The structures of the compounds were elucidated by spectral analyses,including MS and NMR spectral techniques.Results Twenty compounds were isolated from A.fragrans and were identified as 3β-O-β-D-glucopyranosyl-8α-hydroxy-11α,13-dihydrozaluzanin C(1),bergenin monohydrate(2),glucozaluzanin C(3),apigenin 7-O-β-(6′′-methylglucuronide)(4),costunolide-15-β-D-glucopyranoside(5),apigenin-7-O-β-D-glucuronopyranoside(6),luteolin-7-O-β-D-glucuronopyranoside(7),apigenin-6,8-di-C-β-D-glucopyranoside(8),chlorogenic acid(9),1,3-di-O-caffeoylquinic acid(10),3-p-O-trans-coumaroylquinic acid(11),luteolin-7-O-β-D-glucoside(12),luteolin(13),protocatechuic acid(14),5-p-cis-coumaroylquinic acid(15),1-Ocaffeoylquinic acid(16),cryptochlorogenic acid(17),5-p-trans-coumaroylquinic acid(18),3,5-O-dicaffeoylquinic acid(19),and 2′-O-E-caffeoyl-8α-hydroxy-11α,13-dihydro-3β-O-β-D-glucozaluzanin C(20).Conclusion Compounds 2,4—8,11,15—16 and 18 were isolated from the genus Ainsliaea for the first time.

关 键 词：兔耳风属 杏香兔耳风 咖啡酰奎宁酸 芹菜素-7-O-β-D-葡萄糖醛酸苷 木犀草素-7-O-β-D-葡萄糖醛酸苷 芹菜素-6 8-二-C-葡萄糖苷 

分 类 号：R284.1[医药卫生—中药学]