不同粉碎粒度对人参败毒标准汤物理指纹图谱和多指标成分含量的影响  

Effects of different particle sizes on physical fingerprints and contents of multi-index components in Renshen Baidu standard decoction

作　　者：张利恒 陈新[1] 邱智东[1] ZHANG Li-heng;CHEN Xin;QIU Zhi-dong(Key Laboratory for Effective Substances of Genuine Medicinal Material in Changbai Mountain,Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学长白山道地药材药效物质重点研究室,吉林长春130117

出　　处：《中草药》2023年第5期1429-1438,共10页Chinese Traditional and Herbal Drugs

基　　金：重大疫情防治中药方剂储备库建设项目(吉中医药发[2021]11号)。

摘　　要：目的建立人参败毒标准汤的物理指纹图谱和多指标成分含量测定方法,考察不同粉碎粒度对人参败毒散煎出物的影响。方法分别以过10、24、50目筛的人参败毒散制备标准汤,测定盐度(S)、溶解性总固体(total dissolved solids,TDS)、电导率(G)、电位(V)、pH值、动力黏度(μ)、固含量(SC)、表面张力(γ)、折光率(n)9个物理参数,构建其物理指纹图谱,并进行相似度分析(similarity analysis,SA)、层次聚类分析(hierarchical cluster analysis,HCA)、主成分分析(principal component analysis,PCA);通过HPLC-DAD对标准汤中蛇床子素、二氢欧山芹醇当归酸酯、甘草苷、柚皮苷、新橙皮苷的含量进行测定,利用HCA法形成指标成分含量聚类分析图,对物理指纹图谱分析结果进行验证,并考察9个物理参数与5个指标成分的相关性。结果15批人参败毒标准汤未出现异常批次,标准汤S1~S5(过10目筛)、S6~S10(过24目筛)、S11~S15(过50目筛)各聚为一类,不同粉碎粒度批次间的物理指纹图谱相似度均小于0.95,物理参数TDS、G、V、pH、μ、SC、γ、n为人参败毒标准汤物理质量的差异性指标,24目煮散的TDS、G、μ、SC、γ均相对较高;指标成分含量HCA结果与物理指纹图谱分析结果一致,过24目筛的标准汤指标成分含量均高于过10、50目筛的煮散;物理指标TDS、G、V、pH、μ、SC、γ、n与蛇床子素、二氢欧山芹醇当归酸酯、甘草苷、柚皮苷、新橙皮苷的含量之间显著相关。结论粉碎粒度对人参败毒散煎出物的物理属性和指标成分含量具有明显影响,人参败毒散的最佳粉碎粒度为过24目筛,为其质量标准研究和制剂开发提供理论基础。Objective To establish the physical fingerprints of Renshen Baidu standard decoction and the determination method of multi-index components,and investigate the effect of different crushing particle sizes on the decoction of Renshen Baidu powder.Methods The standard decoction was prepared with Renshen Baidu powder that passed through 10,24,and 50 mesh sieve,and nine physical parameters including salinity(S),total dissolved solids(TDS),conductivity(G),potential(V),pH value,dynamic viscosity(μ),solid content(SC),surface tension(γ),refractive index(n)were measured.The physical fingerprints were established,and similarity analysis,hierarchical cluster analysis,and principal component analysis were performed;HPLC-DAD was used to determine the contents of osthol,dihydroeosanthinol-angelate,liquiritin,naringin and neohesperidin in the standard decoction,and the hierarchical cluster analysis method was used to form a cluster analysis chart of the content of the index components,and the results of physical fingerprint analysis were verified.The correlation between nine physical parameters and five index components was investigated.Results There were no abnormal batches of 15 batches of Renshen Baidu standard decoction.The standard decoctions S1—S5(passed through 10 mesh sieve),S6—S10(passed through 24 mesh sieve),and S11—S15(passed through 50 mesh sieve)were grouped together.The similarity of physical fingerprints between batches of different crushed particle sizes is less than 0.95,and the physical parameters TDS,G,V,pH,μ,SC,γ,n are the differences in the physical quality of Renshen Baidu standard decoction,The TDS,G,μ,SC,andγof Renshen Baidu powder through 24 mesh sieve are relatively high;The cluster analysis results of index component content were consistent with the results of physical fingerprint analysis.The index component content of the standard decoction through 24 mesh sieve was higher than the 10 mesh sieve and 50 mesh sieve;The physical indexes TDS,G,V,pH,μ,SC,γ,n were significantly correlated wi

关 键 词：人参败毒散 粉碎粒度 物理指纹图谱 HPLC-DAD 相似度分析 层次聚类分析 主成分分析 蛇床子素 二氢欧山芹醇当归酸酯 甘草苷 柚皮苷 新橙皮苷 

分 类 号：R283.6[医药卫生—中药学]