基于靶向NLRP3炎症小体的金线莲治疗代谢相关脂肪性肝病活性成分发现及机制研究  

Discovery and mechanism of active ingredients of Anoectochilus roxburghii in treatment of metabolic-related fatty liver disease based on targeting NLRP3 inflammasome

作　　者：沈廷明 颜于露 叶希奇 黄春情 吴军军 方芝 王英豪[2] 沈悰祺 吴荔芬[4] SHEN Ting-ming;YAN Yu-lu;YE Xi-qi;HUANG Chun-qing;WU Jun-jun;FANG Zhi;WANG Ying-hao;SHEN Cong-qi;WU Li-fen(Ningde Hospital of Traditional Chinese Medicine,Ningde 352100,China;Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;Shanxi University of Traditional Chinese Medicine,Taiyuan 030024,China;Affiliated Hospital of Putian College,Putian 351100,China)

机构地区：[1]宁德市中医院,福建宁德352100 [2]福建中医药大学,福建福州350122 [3]山西中医药大学,山西太原030024 [4]莆田学院附属医院,福建莆田351100

出　　处：《中草药》2023年第5期1498-1505,共8页Chinese Traditional and Herbal Drugs

摘　　要：目的探索靶向NOD样受体热蛋白结构域相关蛋白3(NOD-like receptor thermal protein domain associated protein 3,NLRP3)炎症小体的中药活性成分发现及机制。方法以金线莲Anoectochilus roxburghii治疗代谢相关脂肪性肝病(metabolic-related fatty liver disease,MAFLD)为例,通过网络药理学方法结合Western blotting研究金线莲靶向调控NLRP3炎症小体的活性成分。结果发现并验证了木犀草素、槲皮素可以抑制核因子-κB(nuclear factor-κB,NF-κB)信号通路的活化,同时验证了木犀草素可以抑制NLRP3炎症小体的激活。结论阐释了金线莲发挥抗MAFLD的物质基础及效应机制,为防治NLRP3炎症小体相关疾病提供了潜在的候选药物,同时为金线莲及相关制剂的有效性评价、质量控制和资源开发提供了科学依据。Objective To explore the active ingredients and mechanism of traditional Chinese medicine targeting NOD-like receptor thermal protein domain associated protein 3(NLRP3)inflammasome.Methods Anoectochilus roxburghii was taken in treatment of metabolic-related fatty liver disease(MAFLD)as an example,network pharmacology combined with Western blotting were used to study the active components of A.roxburghii targeting and regulating the NLRP3 inflammasome.Results Luteolin and quercetin could inhibit the activation of nuclear factor-κB(NF-κB)signaling pathway,and luteolin could inhibit the activation of NLRP3 inflammasome.Conclusion The research results strongly explain the material basis and effector mechanism of anti-MAFLD,provide potential candidates for the prevention and treatment of NLRP3 inflammasome-related diseases,and provide scientific basis for the effectiveness evaluation,quality control and resource development of A.roxburghii and related preparations.

关 键 词：NLRP3炎症小体 代谢相关脂肪性肝病 金线莲 网络药理学 槲皮素 木犀草素 

分 类 号：R285.5[医药卫生—中药学]